This version of that story provides more details about the discovery of
sulfanilamide as the drug's active ingredient and the implications of that fact for the economics of pharmaceutical sales and marketing.
24, 2012) (discussing elixir
sulfanilamide deaths).
The Greiss I) Fluka, UK) Reagent is made up of a 1% solution of
sulfanilamide in 5% phosphoric acid and 0.1% naphthylethylenediamine dihycrochloride in distilled water.
Sulfacetamide,
sulfanilamide, timolol maleate, glacial acetic acid, monobasic sodium phosphate, and phosphoric acid were purchased from Sigma-Aldrich.
Nitrite was determined based on comparison with a standard curve, which was prepared as follows: 10,20, 30, and 40 mL nitrite working standard solutions were added to 50 mL volumetric flasks; 2.5 mL
sulfanilamide reagent was added, mixed, and preceded as above.
With the most prevalent G6PD variants (G6PD Aand G6PD Mediterranean) hemolysis is induced in children and adults by the sudden destruction of older more deficient erythrocytes after exposure to drugs having a high redox potential (including the antimalarial drug primaquine and certain sulfa drugs like
sulfanilamide sulfamethoxazole mafenideor) or to fava beans selected infections or metabolic abnormalities.
Griess reagent was prepared by solving 1g
sulfanilamide in 100 ml phosphoric acid 5% mixed with 0.1g naphtyl ethylene diamine-HCl (NED) in 100 ml distilled water.
Here we have investigated the low-frequency vibrational motions of salicylamide, benzamide, acrylamide, caprolactam, and
sulfanilamide in the solid state by concentrating on the spectral region between 7 and 650 [cm.sup.-1] where crystal lattice vibrations, hydrogen bond bending modes and collective modes, and so forth may occur [26].
Total nitrite was measured at 540 nm absorbance by reaction with Griess reagents (
sulfanilamide and naphthalene-ethylenediamine dihydrochloride).
Briefly, 50 [micro]L of sample and 50 [micro]L of
sulfanilamide solution (1%
sulfanilamide in 5% phosphoric acid) were incubated at room temperature and protected from light for 5-10 minutes.
Nitrite is converted to nitrous acid which then diazotizes
sulfanilamide. The sulfanilamide-diazonium salt then reacts with N-(1-Naphthyl)-ethylenediamine (NED) to form an intense purple-colored diazo compound.
Sulfonamides under study were
sulfanilamide, sulfapyridine, sulfadiazine, sulfisomidine, sulfamethoxypyridazine, sulfamethizole, and sulfamethoxazole (Table 1).
