Abbreviations DIC: N,N'-diisopropylcarbodiimide DIEA: N,N-diisopropylethylamine DMF: N,N-dimethylformamide EDC: 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide HOBt: 1-Hydroxybenzotriazole NHS: N-hydroxy
succinimide PyBOP: Benzotriazol-1-yl-oxytripyrrolidinophosphonium hexafluorophosphate TFA: Trifluoroacetic acid THF: Tetrahydrofuran TIPS: Triisopropylsilane TMSCl: Trimethylsilyl chloride.
Different concentrations of
succinimide were added to the reaction cell with keeping other parameters constant.
We used an N-hydroxy
succinimide esther-PEG (SPA-PEG) for conjugation to the aEPX and aHNL Abs.
Deposit control agents in the combustion chamber are compounds with high temperature stability, mainly
succinimide derivatives [3, 4].
The [H.sup.1] chemical shift scaling factor (0.44) during the evolution period was determined experimentally from the [H.sup.1] chemical shifts of [N.sup.15] enriched
succinimide.
By the similar manner, the other
succinimide esters (3b-d) were prepared as follows.
The first step is the activation of the MUA-carboxyl groups on the surface with a mixture of 0.1 M EDC and 0.1 M NHS to give reactive
succinimide esters.
1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC), N-hydroxyl
succinimide (NHS), and 2-(N-morpholino) ethanesulfonic acid were purchased from Shanghai covalent Chemical Technology Co., Ltd, Shanghai, China.
[17] constructed the adsorption isotherms of
succinimide dispersants with different chain lengths and found the optimum chain length (acting as a lipophilic part) which gives higher adsorption and hence the better coverage of the surface which is a requirement of a stable dispersion.
Metal complexes of
succinimide and phthalimide are of interest due to their unique structural features and potential pharmacological applications [10-11].
N-ethyl-N-(3-dimethyl aminopropyl) carbodiimide hydrochloride (EDC, 26mM) and 10 mM N-hydroxy
succinimide (NHS) were dissolved in 0.1 M 2-(N-morpholino) ethane-sulfonic acid (MES) buffer (pH=8.3).