substrate specificity

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Related to substrate specificity: Lock and Key Model, Lock and Key Theory

sub·strate spec·i·fic·i·ty

the ability of an enzyme to recognize and bind its substrates, typically measured by the Vmax/Km or kcat/Km ratios.


any substance upon which an enzyme acts.

substrate binding site
part of the active site of an enzyme which includes the amino acid residues that come into contact with the substrate.
substrate specificity
range of substrates that can be catalytically converted to product by an enzyme.
suicide substrate
References in periodicals archive ?
Structure of a class HI engineered cephalosporin acylase: comparisons with class I acylase and implications for differences in substrate specificity and catalytic activity, Biochem.
Various substrates were tested to determine substrate specificity of the recombinant NfAXE1 under optimum conditions (40[degrees]C, pH 8).
This broad range of substrate specificity was similar to those of other plant acid phosphatases (Tejera- Garcia et al.
The activity or the apparent substrate specificity is variable with various substances, and so in other words, a PLB could be amenable to the circumstances.
The enzymes belonging to this class vary significantly in substrate specificity, which can correspond to the requirements of ecological niches of the fungi [26].
The phenolic ring on these BFRs superimposes well with that of the acceptor phenol of E2, and is selected for by steric gate residues Phe80 and Phe141, which have been reported to contribute toward substrate specificity (Petrotchenko et al.
Redesigning trypsin: alteration of substrate specificity, catalytic activity and protein conformation.
This should certainly complicate theoretical analysis of the substrate specificity of protein kinase A and the specificity of bisubstrate enzymes in general.
Most plant acid phosphatases show little substrate specificity, exhibit an optimum pH below 6.
The available data about the enzyme substrate specificity towards different substrates vary widely and are different for enzyme preparations from different sources.
Novozymes A/S (Bagsvaerd, Denmark) has patented a method for modifying the substrate specificity of a lipolytic enzyme by making alterations to the amino acid sequence in a defined region of the lipolytic enzyme, so as to increase the level of a desired activity or to decrease the level of an undesired activity.