The authors point out that the hyperhydric state (of newly formed shoots) can also be maintained through several subcultures without too much change, but subculturing
hyperhydric shoots in vitrifying conditions may lead to severe damage, including death of the whole shoot as such, through apparent necrosis of all primary meristems.
Abstract: The effect of successive subculturing of Beauveria bassiana under different in vitro nutritional conditions was evaluated on vegetative growth, conidial production, virulence, tolerance to UV radiation and heat tolerance.
Conidial production, virulence, and stress tolerance of Beauveria bassiana conidia after successive in vitro subculturing
For instance, Metarhizium anisopliae (Metsch.) showed reduced virulence in Tenebrio molitor (Linnaeus) (Coleoptera: Tenebrionidae) and Prl enzyme production after successive subculturing on sabouraud dextrose agar (SDA) medium (Shah et al.
Variable outcomes after successive in vitro subculturing may be associated with inter- and intraspecific variation and the use of monosporic or multisporic cultures.
Successive subculturing was performed on potato dextrose agar (PDA) medium and the medium of A.
Vegetative growth was influenced by a significant interaction between successive subculturing and media.
Subculturing and biochemical tests were conducted in a class II biosafety cabinet.
At laboratory 2, subculturing occurred in a similar manner, but it took place in a class II biosafety cabinet.