subcloning


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sub·clon·ing

(sŭb'klōn-ing),
The process by which a DNA clone is cleaved into smaller pieces and recloned; analysis of overlapping regions of these smaller DNA fragments can confirm the entire sequence of the original DNA clone.

subcloning

a restriction fragment of an original DNA that has been cloned may be further digested with another restriction enzyme and the smaller fragments cloned.
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We used the reverse transcription PCR method and subcloning protocol (online Technical Appendix, https://www.
On the base of recent study experiences, the chance of getting stable hybridoma with high mAb titer was dependent on cell culture medium, appropriate screening and early subcloning methods in aseptic conditions.
Now an encouraging study by scientists at the NIH's National Human Genome Research Institute (NHGRI) suggests that iPSCs do not develop more mutations than cells that are duplicated by subcloning, a technique in which single cells are cultured individually and then grown into a cell line.
To localize the AY-glucosidase gene and shorten the insert for effective analysis of enzymology characterization, subcloning was performed.
Subcloning of the best CHOBRI/Cum2 pools, generated by transfection, increased productivity by 3 to 6-fold yielding clones producing up to 1.
After subcloning ipaB in pBAD/gIII expression vector and its transformation into E.
Leachman for practical advice concerning RT-PCR and subcloning, C.
The amplified product was purified by PCR purification kit following manufacturer's instructions (Qiagen, Germany) and cloned first in the TA cloning vector pTZ57R (Fermantas) followed by subcloning in the yeast shuttle expression vector pGal426 that contains the galactose inducible promoter Gall and uracil synthesizing gene (ura3) as selection marker (12,13).
We then submitted these sequences to Blue Sky Biotech for subcloning and in vitro transcription.
The Morex wild-type rBmy2 enzyme had 16 extra amino acids attached to its N-terminal end, artifacts from subcloning into the expression vector, while the Steptoe wild-type rBmy2 had six extra amino acids on its N-terminal end.