subcloning


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sub·clon·ing

(sŭb'klōn-ing),
The process by which a DNA clone is cleaved into smaller pieces and recloned; analysis of overlapping regions of these smaller DNA fragments can confirm the entire sequence of the original DNA clone.
Farlex Partner Medical Dictionary © Farlex 2012
References in periodicals archive ?
Of that 14-week development time, subcloning and optimal clone identification still typically require four to eight weeks.
This subcloning was further confirmed by subsequent restriction digestion analysis (Figure 1).
pGEX-MED6 was made by subcloning the EcoRI-SalI fragment from pGBT-MED6 into pGEX-2TK (Pharmacia).
After the first cycle of subcloning, five clones stably produced specific antibodies, while the other three clones were determined to be negative (Table 1).
Subcloning of Lipase Genes From Cloning to Expression Vector
On the base of recent study experiences, the chance of getting stable hybridoma with high mAb titer was dependent on cell culture medium, appropriate screening and early subcloning methods in aseptic conditions.
Now an encouraging study by scientists at the NIH's National Human Genome Research Institute (NHGRI) suggests that iPSCs do not develop more mutations than cells that are duplicated by subcloning, a technique in which single cells are cultured individually and then grown into a cell line.
coli DH10B and the vectors pUC18 and pCR2.1 (Invitrogen Life Technologies, USA) were used for subcloning steps.
Subcloning and Detection of Overexpression and Deacetylase Activity of HDAC6.
To localize the AY-glucosidase gene and shorten the insert for effective analysis of enzymology characterization, subcloning was performed.