, a protein produced by the bacteria Streptomyces avidinii, binds biotin with an extremely high affinity, and this binding is utilized in a number of immunoassays, including the assays for thyroid hormone and TSH.
The secondary reagent consisted of biotinylated anti-rabbit antibody (LSAB + System-HRP; Dako[R], Denmark), followed by streptavidin
peroxidase (LSAB + System-HRP, Dako[R], Denmark).
The protocol involved immersion with peroxide enzyme block (Thermo Fisher Scientific, Waltham, MA, USA) for 5 minutes; Power Block Protein block (Biogenex, Fremont, CA, USA) in a 1:10 dilution for 5 minutes; citrate antigen retrieval, pH 6.0 (Biogenex) in a 1:10 dilution for 10 minutes at 120[degrees]C; CD3 (Dako, Agilent Technologies, Santa Clara, CA, USA) for 30 minutes; biotinylated anti-rabbit antibody (Vector Laboratories, Burlingame, CA, USA) in a 1:100 dilution for 10 minutes; [4.sup.+] Streptavidin
horseradish peroxidase label (Biocare Medical, Pacheco, CA, USA) for 10 minutes; and DAB chromogen (Dako, Agilent) for 10 minutes.
agarose and tagged purified Streptavidin
Researchers found that microbubbles conjugated to streptavidin
can be coupled to the surface of a cell, where mechanosensitive Piezo1 ion channels are expressed.
SERS nanostructures produced with gold nanoparticles, functionalized with an optimized SERS reporter (NPCy[(SH).sub.2]), were also functionalized with streptavidin
. The biotin presented on the PS beads and the streptavidin
on the gold nanostructures represent the antigen-antibody pair, used for the identification of cancer cells.
Goat antihuman IgGFc-biotin was from Fitzgerald Industries, Acton, MA, and streptavidin
peroxidase was supplied by Jackson Immunoresearch Laboratories, West Grove, PA.
Horseradish peroxidase (HRP), streptavidin
(SA), glucose oxidase (GOx), 3-(p-hydroxyphenyl)-propanoic acid (HPPA), DNase I, ammonia (25%), silver nitrate, and glucose were purchased from Sinopharm Chemical Reagent Co., Ltd.
Accompanying the improved immunoassays above, the streptavidin-enzyme based ELISA can reduce nonspecific reactions with reagents due to the higher specificity and affinity between streptavidin
and biotin, and it has great potential for detecting trace amounts of BaP.
Louis, MO, USA), MG-l32 from Enzo Life Sciences (Farmingdale, NY, USA), Cy5-conjugated Streptavidin
from Jackson ImmunoResearch Laboratories (West Grove, PA, USA), and Coomassie Stain Solution from Bio-Rad (Hercules, CA, USA).
This method employed a mouse monoclonal antibody (NCL-COX-2) of Vision Biosystems, NovocastraTM as primary antibody and secondary antibody of Ultra Tech HRP (DAB) Streptavidin
Biotin Detection System from Beckman Coulter was used.
Owing to streptavidin
's extraordinarily high affinity for biotin [dissociation constant ([K.sub.d]) of [10.sup.-14] mol/L] and binding under a wide variety of chemical conditions, biotin-streptavidin is a popular component of assay architecture for many molecular tests and immunoassay platforms.