Using the stereotaxic instrument
, it was possible to avoid any other interference to the measurement of IOP.
The head of each rat was horizontally fixed onto the mouse stereotaxic instrument
and the three-dimensional coordinates of the right SNc and VTA were determined in reference to the atlas by Ming and Yun. A total of 10 [micro]g of 6-OHDA was injected at each point, with the equivalent dose of NS injected into rats in the normal + NS group.
Mukul Jain, Senior Vice President, Zydus Research Centre by providing Stereotaxic instrument
to our institute and Dr.
For treatment of K252a, the rats were anesthetized with injection of pentobarbital sodium solution and placed in a stereotaxic instrument
. One hole was made on the opposite side of injury for injection at coordinates -1.0 mm anterior to posterior (AP) bregma and 1.5 mm mid to lateral (ML) and -3.8 mm dorsal to ventral (DV) dura according to the stereotaxic atlas of Paxinos and Watson (Paxinos, 1996).
For stereotaxic lesions, anesthetized rats were placed in the stereotaxic instrument
(Narishige SR-9, Tokyo, Japan), and the skull was exposed under sterile conditions.
The anesthetized animals were mounted on stereotaxic instrument
(INCO, India Ltd.) equipped with a custom-made ear bar that prevents the damage of the tympanic membrane.
The rat was mounted in the prone position on a stereotaxic instrument
(Kopf Instruments, Tujunga, California, USA).
The Lab Standard Stereotaxic Instrument
is ideal for procedures with small animals.
Adult male rats were anesthetized (65 mg/kg sodium pentobarbital, intraperitoneally) and placed in a Kopf model 900 stereotaxic instrument
(David Kopf Instruments, Tujunga, CA).
for the needle biopsy of the mamma.