Anatomical definitions: The boundaries of the hippocampal structure including the dentate gyrus (DG) and the Cornu Ammonis (CA) regions were discreetly defined at all levels of sectioning according to the stereotaxic
coordinates of the Paxinos and Franklin's Mice Brain Atlas.
Using the stereotaxic
instrument, it was possible to avoid any other interference to the measurement of IOP.
Twelve rats were anesthetized and placed in stereotaxic
head frame on a heating pad.
Then, two groups of animals (n = 6, each group) were anesthetized with a mix of ketamine-xylazine solution (1 ml/kg) and placed on a stereotaxic
apparatus (KOPF Mod 5000, USA); then, stereotaxic
injections were made by using a 10 [micro]l Hamilton syringe (model 701) coupled in manual microinjector with a velocity of 106 nl per revolution (Sutter Instrument USA).
coordinates  were referred to select brain slices containing the auditory cortex.
Rats were deeply anesthetized with isoflurane (3% for induction, 1-2% for maintenance) in a 70 : 30 mixture of nitrous oxide and oxygen using an isoflurane vaporizer (VetEquip Inc., Livermore, CA) and were placed in a stereotaxic
frame (Kopf Instruments, Tujunga, CA).
For inducing DLB in the rat model, rats were randomly divided into three groups and underwent stereotaxic
brain surgery on day 0 as described in our previous reports [8, 27].
During the experiment, the animals were fixed under the stereotaxic
instruments (Takahashi Company, Japan) with blunt ear bars, and a small craniotomy was performed.
A small hole was drilled in the skull over the CnF according to the stereotaxic
coordinates of the Rat Brain in Stereotaxic
Coordinates by Paxinos and Watson (7.6-8.5 mm caudal to the bregma, 1.7-2.2 mm lateral to the midline suture, and 5.5-6.2 mm ventral from the bregma).
The anesthetized animals were fixed in a stereotaxic
These surgical techniques includes CT guided stereotaxic
aspiration, simple aspiration of ICH through a burr hole and open craniotomy each having their own merits and demerits.7 The aim of this study was to assess the risks and benefits of surgical treatment (Open Craniotomy) of Intra-cerebral hematoma (ICH) in our set up and consider the implications of the results on current clinical practice and future research endeavors.
Their brains are larger when compared with rodents, enabling conventional neurosurgery, stereotaxic
neurosurgery, and surgical implantation of devices intended for human use (Lind et al., 2007; Nielsen et al., 2009; Conrad et al., 2012; Dolezalova et al., 2014).