standard curve


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standard curve

a graphic plot of tracer binding versus the known concentration of test substances in a set of standards usually prepared by serial dilution or incremental addition.
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The quantitative properties of qPCR are based upon running unknown samples alongside a standard curve of known copy numbers of the target DNA (Larionov et al.
Because this information was not provided by Diasorin, we created our own standard curve for Liaison.
Virus titers were extrapolated from RT-PCR results by comparison with a standard curve generated from serial dilutions of a VEEV stock quantified by plaque assay.
2] with catalase was determined from a standard curve.
Absorbance was measured at 420 nm after 30 min and plotted against concentration to made standard curve, from where unknown concentration of phosphorous was determined (Table-4).
The beads are run on the cytometer on the same day and at the same instrument settings as cells to generate a standard curve relating channel value to a standardized fluorescence-intensity unit--molecules of equivalent soluble fluorochrome (MESF).
Quantification was performed using a standard curve prepared by serial dilutions of recombinant ZAG added to 50 g/L BSA in phosphate-buffered saline as a surrogate serum matrix.
This allowed the researchers to use advanced analysis techniques to search for the genes that seemed to be sensitive to aging, and create a standard curve, or timeline, that described the way they changed.
The standard curve of flavonoids content was analyzed using rutin as the standard at 400 nm, using an ultraviolet (UV) spectrophotometer, and the standard curve was obtained, C = 0.
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