Identification of Cryptococcus neoformans isolates using Staib agar without creatinine.
Staib agar has been widely used for identification of yeasts from the genus Cryptococcus and other fungi.
Key words: Staib agar, Guizotia abyssinica, Cryptococcus neoformans, creatinine.
Staib agar medium has been widely used for isolation and identification of yeasts from the genus Cryptococcus.
Initially, Staib agar was being prepared with the traditional method, but further on in the study there was a failure in the supply of some of the compounds, especially creatinine.
The aim of this study was to assess the usefulness of Staib agar without creatinine for the identification of C.
Anette Fothergill) were used to assess their development in Staib agar with and without creatinine.
The same strains were tested for phenoloxidase production, culturing in standard and modified Staib agar.
In standard Staib agar, colonies were light brown color, without green diffusible pigment; in Staib without creatinine, colonies were dark brown.
dubliniensis strains were inoculated on standard Staib agar and Staib agar without creatinine.
The most common are Sabouraud agar, Malt agar, Cornmeal agar, Potato-dextrose agar, and Staib agar (6, 16, 17).
Staib in 1962, developed Staib agar, using Guizotia abyssinica seeds (1).