oocsyts can exist in the environment and are resistant to many disinfectants, including the chlorine used in municipal water-treatment plants.
The species determination was done morphometrically, as sporulated
coccidian oocysts differ in size and morphology.
Isolation of the sporulated
oocysts: The sporulated
oocysts were separated by Zinc sulphate floatation technique (Ryley et al., 1976).
BALB/c male mice were infected orally with [10.sup.4] sporulated
oocysts as previously described .
1--Photomicrographs of sporulated
oocysts of Eimeria vilasi.
Oral-fecal transmission in a contaminated environment having sporulated
oocysts is the most common infection route.
Remaining twenty birds in each group were challenged with 65000-70000 oocysts (sporulated
) of mixed Eimeria species (local isolate) at 14th day post-inoculation of polysaccharides.
The plugs were inoculated with a loopful of well sporulated
cultures and incubated in a moist chamber at 25[degrees]C.
Successive in vivo passages of the fungal strains were made by placing 100 live and healthy insects in contact with dead and contaminated insects, the bodies of which were covered with the sporulated
After 13 days of infection of the sunflower inbred line 'FU', the zoosporangia and sporangiophores suspensions for 20 single zoosporangium isolates were obtained by grouping all sporulated
cotyledons in a small container and adding 1 ml of physiological water for each cotyledon (9g NaCl+1L sterilized water).
Linalool (75) and a-terpineol (76) were the main products obtained from nerol by sporulated
surface cultures along with some minor products, where as geraniol (77) was converted predominantly to linalool (75) also resulting in higher yields (Scheme 28).
Inoculum preparationA volume of 7 mL of sterile distilled water was transferred to a sporulated
(7days old) PDA slant culture.