Mycorrhizal association in gametophytes and sporophytes
of fern Pteris vittata (Peridaceae) with Glomus intraradices.
Spores from four different sporophytes
of each location were mixed and sowed in petri plates six cm in diameter with mineral agar medium (Dyer 1979).
The ability to self-fertilise produces a higher number of sporophytes
in monoicous as opposed to dioicous species.
At 28 days, all sporophytes
were washed thoroughly in distilled water, dewatered and then oven dried at 50[degrees]C until weight was constant.
Inoculation in tanks facilitates the adhesion and development of more than 10 sporophytes
per linear cm of rope and the latter possibility of obtaining samples for comparisons of morphological development.
The outside of the rhizome mass of the Polytaenium supported gametophytes and young sporophytes
of additional plants of Polytaenium, showing that the site was favorable for its growth and establishment.
However, for each time interval we had a minimum of two subreplicates from at least 50% of the sporophytes
to calculate the mean values.
In the first method the whole 'head' of the kelp sporophyte
is cut off between the bulb at the top of the stipe and the primary blade.
Plants have two generations: sporophyte
Plants are very fertile as the sporophytes
are found in abundance.
Our research shows that sporophytes
as well as gametophytes of P.
The fact that early fern gametophytes were not preserved in the fossil record allows the conclusion that comparable delicate sporophytes
had undergone the same fate.