spoligotyping

spoligotyping

(spol?i-go-tip'ing) [Fm. sp(acer) oligtyping]
The use of the polymerase chain reaction to identify pathogens, such as Mycobacterium tuberculosis, in laboratory specimens. It relies on the detection of unique spans of repeated DNA sequences found between the active genes of the pathogen. Culture-based methods of identifying mycobacteria are slow, often taking as long as 4 to 6 weeks to identify microorganisms in sputum or blood. DNA fingerprinting requires considerable technical expertise. Spoligotyping is simpler, more economical, and a more efficient means of identifying slowly growing microorganisms.
Synonym: spacer oligotyping
References in periodicals archive ?
Three months after transplantation, and before identification of cases with a matching genotype based on spoligotyping (a polymerase chain reaction [PCR]--based method) and 24-locus variable-number tandem repeat of mycobacterial interspersed repetitive units (VNTR-MIRU) (a PCR method that analyzes specific regions of the genome), the recipient developed a persistent cough and fatigue.
A snapshot of the biodiversity and clustering of Mycobacterium tuberculosis in Oman using spoligotyping.
Genotyping of the Mycobacterium tuberculosis complex using MIRUs: association with VNTR and spoligotyping for molecular epidemiology and evolutionary genetics.
A realizacao de genotipagem por Spoligotyping em uma unidade prisional do Rio Grande do Sul demonstrou que a maior parte dos isolados clinicos (87,3%) apresentava genotipos identicos, sendo, portanto, identificados como pertencentes a algum cluster.
Bacterial DNA was subjected to macroarray-based spoligotyping (8) and whole-genome sequencing on the MiSeq platform (Illumina, San Diego, CA, USA).
Assessment of an optimized mycobacterial interspersed repetitive-unit-variable-number tandem-repeat typing system combined with spoligotyping for population-based molecular epidemiology studies of tuberculosis.
Finally, 20 [micro]L of supernatant from the resulting supernatant was aliquotted to carry out PCR reaction for spoligotyping and the Hain test as previously described [10,11].
IS6110-RFLP and spoligotyping of Mycobacterium tuberculosis isolates in Iran.
Characterization of Mycobacterium tuberculosis complex DNAs from Egyptian mummies by spoligotyping.
Spoligotyping of Mycobacterium tuberculosis isolates from Pakistan reveals predominance of Central Asian Strain1 and Beijing isolates.