spoligotyping

spoligotyping

(spol?i-go-tip'ing) [Fm. sp(acer) oligtyping]
The use of the polymerase chain reaction to identify pathogens, such as Mycobacterium tuberculosis, in laboratory specimens. It relies on the detection of unique spans of repeated DNA sequences found between the active genes of the pathogen. Culture-based methods of identifying mycobacteria are slow, often taking as long as 4 to 6 weeks to identify microorganisms in sputum or blood. DNA fingerprinting requires considerable technical expertise. Spoligotyping is simpler, more economical, and a more efficient means of identifying slowly growing microorganisms.
Synonym: spacer oligotyping
References in periodicals archive ?
tuberculosis isolates by using spoligotyping and large-sequence polymorphism (LSP) as described previously (5).
Spoligotyping (a rapid, polymerase chain reaction-based method for genotyping strains of the M.
tuberculosis such as IS6110-RFLP, MIRU-VNTR, and spoligotyping. All these techniques are quite complex, time-consuming and expensive (Guillen-Nepita et al., 2013; Jonsson et al., 2014).
All samples were characterized by phonotypical drug susceptibility test, genotypic drug resistant test (line probe assay) and spoligotyping analysis using 'TB-SPRINT' micro bead assay.
Spoligotyping. Spoligotyping--commonly used to differentiate Mycobacterium tuberculosis complex strain--is based on polymorphisms of the chromosomal direct repeat (DR) locus, which contains a variable number of short DRs interspersed with nonrepetitive spacers [56, 57].
Three months after transplantation, and before identification of cases with a matching genotype based on spoligotyping (a polymerase chain reaction [PCR]--based method) and 24-locus variable-number tandem repeat of mycobacterial interspersed repetitive units (VNTR-MIRU) (a PCR method that analyzes specific regions of the genome), the recipient developed a persistent cough and fatigue.
A snapshot of the biodiversity and clustering of Mycobacterium tuberculosis in Oman using spoligotyping. Int J Tuberc Lung Dis 2010 Aug; 14(8):9941000.
Genotyping methods, such as spoligotyping and variable number tandem repeats typing (VNTR), have been commonly used to identify MTB genotypes, on the basis of polymerase chain reaction (PCR) [6, 7].
Among them, spoligotyping has the advantage of being relatively simple, inexpensive, and generally sufficient as a first approach of molecular epidemiology of TB [8].
Spoligotyping was performed using commercial kit manufactured by Isogen Bioscience BV (Maarssen, Netherlands) [4].
Se recolectaron muestras de esputo para la deteccion del bacilo a traves de metodologias convencionales (baciloscopia y cultivo) y moleculares (Spoligotyping).