tuberculosis isolates by using spoligotyping
and large-sequence polymorphism (LSP) as described previously (5).
(a rapid, polymerase chain reaction-based method for genotyping strains of the M.
is the most widely used method for genotyping of M.
tuberculosis such as IS6110-RFLP, MIRU-VNTR, and spoligotyping
. All these techniques are quite complex, time-consuming and expensive (Guillen-Nepita et al., 2013; Jonsson et al., 2014).
All samples were characterized by phonotypical drug susceptibility test, genotypic drug resistant test (line probe assay) and spoligotyping
analysis using 'TB-SPRINT' micro bead assay.
. Spoligotyping--commonly used to differentiate Mycobacterium tuberculosis complex strain--is based on polymorphisms of the chromosomal direct repeat (DR) locus, which contains a variable number of short DRs interspersed with nonrepetitive spacers [56, 57].
Three months after transplantation, and before identification of cases with a matching genotype based on spoligotyping
(a polymerase chain reaction [PCR]--based method) and 24-locus variable-number tandem repeat of mycobacterial interspersed repetitive units (VNTR-MIRU) (a PCR method that analyzes specific regions of the genome), the recipient developed a persistent cough and fatigue.
A snapshot of the biodiversity and clustering of Mycobacterium tuberculosis in Oman using spoligotyping
. Int J Tuberc Lung Dis 2010 Aug; 14(8):9941000.
Genotyping methods, such as spoligotyping
and variable number tandem repeats typing (VNTR), have been commonly used to identify MTB genotypes, on the basis of polymerase chain reaction (PCR) [6, 7].
Among them, spoligotyping
has the advantage of being relatively simple, inexpensive, and generally sufficient as a first approach of molecular epidemiology of TB .
was performed using commercial kit manufactured by Isogen Bioscience BV (Maarssen, Netherlands) .