Three months after transplantation, and before identification of cases with a matching genotype based on spoligotyping
(a polymerase chain reaction [PCR]--based method) and 24-locus variable-number tandem repeat of mycobacterial interspersed repetitive units (VNTR-MIRU) (a PCR method that analyzes specific regions of the genome), the recipient developed a persistent cough and fatigue.
A snapshot of the biodiversity and clustering of Mycobacterium tuberculosis in Oman using spoligotyping
Genotyping of the Mycobacterium tuberculosis complex using MIRUs: association with VNTR and spoligotyping
for molecular epidemiology and evolutionary genetics.
La metodologia de Spoligotyping
se realizo segun lo descrito por Kremer en el 2002.
A realizacao de genotipagem por Spoligotyping
em uma unidade prisional do Rio Grande do Sul demonstrou que a maior parte dos isolados clinicos (87,3%) apresentava genotipos identicos, sendo, portanto, identificados como pertencentes a algum cluster.
Bacterial DNA was subjected to macroarray-based spoligotyping
(8) and whole-genome sequencing on the MiSeq platform (Illumina, San Diego, CA, USA).
Assessment of an optimized mycobacterial interspersed repetitive-unit-variable-number tandem-repeat typing system combined with spoligotyping
for population-based molecular epidemiology studies of tuberculosis.
Finally, 20 [micro]L of supernatant from the resulting supernatant was aliquotted to carry out PCR reaction for spoligotyping
and the Hain test as previously described [10,11].
of Mycobacterium tuberculosis in anthracotic bronchitis.
IS6110-RFLP and spoligotyping
of Mycobacterium tuberculosis isolates in Iran.
Characterization of Mycobacterium tuberculosis complex DNAs from Egyptian mummies by spoligotyping
of Mycobacterium tuberculosis isolates from Pakistan reveals predominance of Central Asian Strain1 and Beijing isolates.