Three months after transplantation, and before identification of cases with a matching genotype based on
spoligotyping (a polymerase chain reaction [PCR]--based method) and 24-locus variable-number tandem repeat of mycobacterial interspersed repetitive units (VNTR-MIRU) (a PCR method that analyzes specific regions of the genome), the recipient developed a persistent cough and fatigue.
A snapshot of the biodiversity and clustering of Mycobacterium tuberculosis in Oman using
spoligotyping.
Genotyping of the Mycobacterium tuberculosis complex using MIRUs: association with VNTR and
spoligotyping for molecular epidemiology and evolutionary genetics.
La metodologia de
Spoligotyping se realizo segun lo descrito por Kremer en el 2002.
A realizacao de genotipagem por
Spoligotyping em uma unidade prisional do Rio Grande do Sul demonstrou que a maior parte dos isolados clinicos (87,3%) apresentava genotipos identicos, sendo, portanto, identificados como pertencentes a algum cluster.
Bacterial DNA was subjected to macroarray-based
spoligotyping (8) and whole-genome sequencing on the MiSeq platform (Illumina, San Diego, CA, USA).
Assessment of an optimized mycobacterial interspersed repetitive-unit-variable-number tandem-repeat typing system combined with
spoligotyping for population-based molecular epidemiology studies of tuberculosis.
Finally, 20 [micro]L of supernatant from the resulting supernatant was aliquotted to carry out PCR reaction for
spoligotyping and the Hain test as previously described [10,11].
Spoligotyping of Mycobacterium tuberculosis in anthracotic bronchitis.
IS6110-RFLP and
spoligotyping of Mycobacterium tuberculosis isolates in Iran.
Characterization of Mycobacterium tuberculosis complex DNAs from Egyptian mummies by
spoligotyping.
Spoligotyping of Mycobacterium tuberculosis isolates from Pakistan reveals predominance of Central Asian Strain1 and Beijing isolates.
