spoligotyping

(spol?i-go-tip'ing) [Fm. sp(acer) oligtyping]

The use of the polymerase chain reaction to identify pathogens, such as Mycobacterium tuberculosis, in laboratory specimens. It relies on the detection of unique spans of repeated DNA sequences found between the active genes of the pathogen. Culture-based methods of identifying mycobacteria are slow, often taking as long as 4 to 6 weeks to identify microorganisms in sputum or blood. DNA fingerprinting requires considerable technical expertise. Spoligotyping is simpler, more economical, and a more efficient means of identifying slowly growing microorganisms.

Synonym: spacer oligotyping

References in periodicals archive ?

tuberculosis isolates by using spoligotyping and large-sequence polymorphism (LSP) as described previously (5).

Mixed Mycobacterium tuberculosis Lineage Infection in 2 Elephants, Nepal

Spoligotyping (a rapid, polymerase chain reaction-based method for genotyping strains of the M.

Tuberculosis transmission in a hospitalised neonate: Need for optimised tuberculosis screening of pregnant and postpartum women

Spoligotyping is the most widely used method for genotyping of M.

Molecular Characterization and Drug Susceptibility of Mycobacterium Bovis Isolated from Cattle in Xinjiang, China

tuberculosis such as IS6110-RFLP, MIRU-VNTR, and spoligotyping. All these techniques are quite complex, time-consuming and expensive (Guillen-Nepita et al., 2013; Jonsson et al., 2014).

Genetic Diversity of Mycobacterium tuberculosis Complex Prevailing In Khyber Pakhtunkhwa, Pakistan

All samples were characterized by phonotypical drug susceptibility test, genotypic drug resistant test (line probe assay) and spoligotyping analysis using 'TB-SPRINT' micro bead assay.

Molecular Characterization and Spoligotyping Analysis of Multi Drug Resistant Mycobacterium tuberculosis from a High TB Endemic Area of Pakistan

Spoligotyping. Spoligotyping--commonly used to differentiate Mycobacterium tuberculosis complex strain--is based on polymorphisms of the chromosomal direct repeat (DR) locus, which contains a variable number of short DRs interspersed with nonrepetitive spacers [56, 57].

Cutaneous Tuberculosis: Clinicopathologic Arrays and Diagnostic Challenges

Three months after transplantation, and before identification of cases with a matching genotype based on spoligotyping (a polymerase chain reaction [PCR]--based method) and 24-locus variable-number tandem repeat of mycobacterial interspersed repetitive units (VNTR-MIRU) (a PCR method that analyzes specific regions of the genome), the recipient developed a persistent cough and fatigue.

Solid organ transplant-transmitted tuberculosis linked to a community outbreak--California, 2015

A snapshot of the biodiversity and clustering of Mycobacterium tuberculosis in Oman using spoligotyping. Int J Tuberc Lung Dis 2010 Aug; 14(8):9941000.

Epidemiology of drug-resistant tuberculosis in a tertiary Care Center in Oman, 2006-2015

Genotyping methods, such as spoligotyping and variable number tandem repeats typing (VNTR), have been commonly used to identify MTB genotypes, on the basis of polymerase chain reaction (PCR) [6, 7].

Difference in Antibody Responses to Mycobacterium tuberculosis Antigens in Japanese Tuberculosis Patients Infected with the Beijing/Non-Beijing Genotype

Among them, spoligotyping has the advantage of being relatively simple, inexpensive, and generally sufficient as a first approach of molecular epidemiology of TB [8].

First Insight into a Nationwide Genotypic Diversity of Mycobacterium tuberculosis among Previously Treated Pulmonary Tuberculosis Cases in Benin, West Africa

Spoligotyping was performed using commercial kit manufactured by Isogen Bioscience BV (Maarssen, Netherlands) [4].

First Insight into the Molecular Epidemiology of Mycobacterium tuberculosis Isolates from the Minority Enclaves of Southwestern China