The publication highlights the anti-tumor activity of H3B-8800 in several in vivo models, including patient-derived xenografts of hematological malignancies with recurrent mutations in RNA splicing factor genes that comprise the spliceosome
We found that they're heavily involved in the components of three critical cell structures: the spliceosome
, the ribosome-which use genetic information to construct proteins for the cell-and the proteasome, which is a vital protein machine that disposes of unneeded protein material.
Among specific topics are RNA elements involved in splicing, the expression and purification of splicing proteins, array analysis, stable cell lines with splicing reporters, and changing signals to the spliceosome
As Bhattacharya notes, the spliceosome
has at least 150 proteins needed in the job of "editing" messenger RNA before it even gets to the ribosome to be translated into protein.
In the process of eukaryotic transcription, LSM proteins associate with small nuclear RNA to generate the spliceosome
complex which helps facilitate the process of RNA splicing.
When this site is absent, the spliceosome
is unable to recognize the exon-intron boundary.
Selective Inhibition of Topoisomerase I and Various Steps of Spliceosome
Assembly by Diospyrin Derivatives.
RNAsplicing is executed by a complex of proteins and small nuclearRNAs(snRNAs) called the spliceosome
We know that as many as 90 structures (epitopes) of the spliceosome
protein autoantigens (Sm and nRNP) can be recognized by autoantibody (in both man and animal models), (6,7) while more than 20 epitopes are found in the 60 kD Ro system.
Specific topics addressed include riboswitches, catalytic strategies of self-cleaving ribozymes, the synthesis of protein, the world of small nuclear ribonucleoproteins, spliceosome
structure and function, the biology of short RNAs, large noncoding RNAs in mammalian gene dosage regulation, automated in vitro selection and microarray applications for functional RNA sequences, and the prediction of RNA secondary structure.
Sharon Wolf of the Chemical Research Support Department at the Weizmann Institute, have created a 3-D image of the spliceosome
'machine' where RNA splicing takes place, which is situated in the nucleus of a cell.
excises or cuts the non-coding regions (introns) and splices together the coding regions (exons).