's job is to modify the message relayed from DNA by clipping genetic bits in such a manner that they are acceptable for translation into protein.
In the vertebrate nucleus, splicing is performed by the spliceosome
, a ribosome-sized complex that contains mostly protein, but also includes, at various stages of the reaction, five snRNAs.
Then the lariat is joined by spliceosome
and the introns are removed to form a circRNA.
Loss of immune tolerance to components of large RNP moieties being part of spliceosomes
or ribosomes seems to be characteristic for distinct SARD .
Term KEGG pathway p value hsa05220 Chronic myeloid leukemia 0.004 hsa05215 Prostate cancer 0.008 hsa04810 Regulation of actin cytoskeleton 0.011 hsa05221 Acute myeloid leukemia 0.014 hsa04110 Cell cycle 0.024 hsa03040 Spliceosome
0.024 hsa05212 Pancreatic cancer 0.025 hsa04010 MAPK signaling pathway 0.029 hsa04350 TGF-beta signaling pathway 0.041
, a large ribonucleoprotein (RNP) complex that contains small nuclear RNP particles, snRNP, and other numerous protein factors including RNA helicases and protein kinases are involved in the splicing process in plants [21, 22].
Intron splicing accomplished by the spliceosome
is closely related with four cis-acting elements, that is, the 5' splicing sites (5SS), the 3' splicing sites (3SS), the poly-pyrimidine tract (PPT), and the branch point (BP) .
Traditional models of how alternative splicing is regulated involve auxiliary splicing factors that bind to the premRNA and enhance or repress the ability of the spliceosome
to recognize particular splice sites (20).
These technologies can help to unweave the complex network of functional interactions within the spliceosome
and of the spliceosome
with regulatory factors, exhaustively map the contribution of regulatory sequences and be used to investigate, with unprecedented detail, mechanisms of regulation for essentially any regulator or alternative splicing event operating in a particular cell line.
Vazquez, "The crosstalk between plant microRNA biogenesis factors and the spliceosome
," Plant Signaling & Behavior, vol.
These new disease-associated mutations occurred in genes coding for proteins that function as signaling molecules, transcription factors, epigenetic regulators and elements of the spliceosome
complex, a component of cells DNA transcription machinery.
Mass spectrometry and EST-database searching allows characterization of the multi-protein spliceosome