Nonstandard abbreviations: NSCLC, non-small-cell lung cancer; TNM, tumor-node-metastasis; KLK, kallikrein-related peptidase; KLK8-T1, KLK8 mRNA splice variant
type 1; RT-PCR, reverse-transcription PCR; OS, overall survival; RR, relative risk.
Lamins A and C are coded by a single developmentally regulated gene designated LMNA; lamin C is a splice variant
and lacks the carboxyl terminus present in lamin A.
In conclusion, our study shows for the first time the splice variant
profile of VEGF in a quantitative aspect in NSCLC.
Our main purpose was to investigate the potential prognostic value of TIMP1 mRNA and a newly discovered splice variant
to gain more knowledge on the biology of TIMP-1 in breast cancer.
M2 PHARMA-August 27, 2013-Researchers identify breast cancer gene splice variant
that may leave patients undertreated(C)2013 M2 COMMUNICATIONS
We placed 1 [micro]L of cDNA into a 9-[micro]L reaction mixture that contained 0.1 [micro]L of Taq DNA polymerase (5 U/[micro]L, Platinum DNA Polymerase; Invitrogen),1 [micro]L of the supplied 10x PCR buffer, 0.5 [micro]L of Mg[Cl.sub.2] (50 mmol/L), 0.2 [micro]L of dNTPs (10 mmol/L; Fermentas), 0.15 [micro]L of bovine serum albumin (10 g/L; Serva), 0.5 [micro]L of the appropriate sense primer for each splice variant
, 0.5 [micro]L of the corresponding antisense primer (3 [micro]mol/L), and 1 [micro]L of the TagMan probe (3 [micro]mol/L); finally, DEPC-[H.sub.2]O was added to a final volume of 10 [micro]L.
Similarly, mutations within introns can influence splice site selection and lead to inappropriate expression of particular splice variant
isoforms of a gene which is otherwise expressed at an overall appropriate level.
The detection of this splice variant
by RT-PCR in RNA isolated from FFPE tissue from patients with lung cancer is now standard in our institution and we are now setting up this technique in cytology smears.
Recently, however, promising studies have been published, investigating the role of androgen receptor splice variant
7 (AR-V7) in prostate cancer (7, 8).
In vitro and in vivo studies have shown that splice variant
1 (SV1) of the GHRH receptor, which is widely expressed in non-pituitary tissues and cancers, can mediate the proliferative effects of GHRH [10,12].
However, whilst an SUR2 splice variant
lacking exon 17 has been identified in mice which had a 2-fold reduction in sensitivity to ATP compared to the full-length isoform (15), a study using rat SUR1[DELTA]17 found no differences in sensitivity to MgATP between the mutant and wild-type channels (6).
The aim of the present study therefore, was to investigate whether the expression of the anti-angiogenic VEGF splice variant
; VEGF165b could be altered in human breast cell lines exposed to different classes of antiretroviral drugs, individually and in combination.