A study with cyclophosphamide as an antigen has revealed that okra polysaccharides increased spleen index, splenocyte
proliferation, and cytokines secretion .
We demonstrate that in early stages of acute phase, [IL-4.sup.-/-] knockout animals presented milder myocarditis with lower IFN-[gamma] production and higher in situ IL-10 production, despite Th1 cells increase in splenocyte
Cytokines detected by ELISA in splenocyte
culture from immunized mice after stimulation with tachyzoite Toxoplasma Lysate Antigens (TLA) Number Immunization regimen (IFN-[gamma], pg/ml) Sig.
Moreover, the combined immunotherapy significantly increased the secretion of IFN-[gamma] and conversely diminished the secretion of IL-4 and TGF-[beta] in the splenocyte
population compared to the splenocytes
from the other groups.
cultures were prepared as described previously.[sup] Splenocytes
were harvested from EAE mice (2–3 scores) 5 days after MOG immunization.
apoptosis in Plasmodium berghei ANKA infection: possible role of TNF-a and TGF-b.
For the natural killer cell and splenocyte
cell, RPMI 1640 medium was used instead of DMEM.
A mycelial lectin from Aspergillus nidulans microfungi induced an increase of NO production, and IFN[gamma] and IL-6 levels also enhanced in splenocyte
cultures from treated Swiss albino mice groups.
Table 2: Splenocyte
proliferative responses and flow cytometry analysis of T cell subclasses in spleen cells between the immunization and the controls.
(2008) Briefly, the splenocyte
suspension in RPMI-1640 medium (4 x [10.sup.5] cells/ml), was preincubated in 96 well plates (200 [micro]l/well) with or without mitogens Con-A or LPS at a concentration of 5 and 10 [micro]g/ml, respectively) at 37 [degrees]C for 72 h in a 5% C[O.sub.2] incubator.
Nigella sativa modulates splenocyte
proliferation, Th1/Th2 cytokine profile, macrophage function and NK anti-tumor activity.
The results demonstrated that CPP could promote the splenocyte
proliferation, increase the activity of acid phosphatase in peritoneal macrophages and strengthen peritoneal macrophages to devour neutral red in vitro.