This suggested bacterial replication taking place within the perfused spleen, and confocal microscopy confirmed the formation of clusters of bacteria within splenic cells
at later time-points (Fig.
The exact mechanism through which splenosis occurs in not clearly understood; the most plausible hypothesis is that splenic cells
reach adjacent organs after capsule rupture; the preferential sites of splenosis are stomach, small bowel, peritoneum, and colon (1,2).
To determine the changes in the percentage of T cells that respond to antigenic stimulus, we performed immunophenotypic characterization and quantified the mononuclear splenic cells
. Our findings indicated a clear predominance of CD4 (+) T cells in all immunized groups studied.
Human splenic cells
were isolated from healthy donors at the Institute of Organ Transplantation, Tongji Hospital (TJ-IRB20160601).
To determine the role of B cells in the induction of the antibody response against P3 mAb, B-1a peritoneal cells and B-2 splenic cells
were magnetically purified as previously described in Section 2.3.
Effects of cytokine-specific mRNA in primary splenic cells
Protein content was moderately decreased in the splenic cells
of mice infected with P.
The immuno-modulation of AG was tested using flow cytometry analyses of splenic cells
isolated from CT26-bearing mice.
Isolation and Cultivation of Splenic Cells
. In order to activate cytokines synthesis and secretion we cultivated [10.sup.6]/mL spleen cells in 1 mL of culture medium with concanavalin A (5mkg/mL) for 20 hours at 37[degrees]C and 5% C[O.sub.2] in 24-well cultured plates.
The resulting suspension of splenic cells
was washed and resuspended in PRMI 1640 medium at a concentration of 2 x [10.sup.6]/mL.
There is currently another theory stating that an abnormal development of the cranial suspensory ligament of testis causes an abnormal involution resulting in cryptorchism and colonization of the abnormal ligament by splenic cells
Surprisingly, splenosis has been traced in the brain, indicating haematogenous spread of the splenic cells
. (44) Port site splenosis was also reported by Kumar and Borzi.