spheroplast


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spheroplast

 [sfēr´o-plast]
a bacterial, yeast, or fungal cell that results after partial removal of the rigid cell wall, which forms a membrane-bound cell with a spherical shape that is dependent for its integrity on an isotonic or hypertonic medium.

sphe·ro·plast

(sfē'rō-plast),
A bacterial cell from which the rigid cell wall has been incompletely removed. The bacterium loses its characteristic shape and becomes round.
[sphero- + G. plastos, formed]

spheroplast

(sfîr′ə-plăst′, sfĕr′-)
n.
A bacterial cell whose cell wall has been degraded, often by the action of an antibiotic or a lysozyme, causing the cell to assume a spherical shape.

spheroplast

gram-negative bacterial cell deprived of its cell wall, but retaining an intact plasma membrrane. See also protoplast.
References in periodicals archive ?
The release of enzymes from Escherichia coli by osmotic shock and during the formation of spheroplasts.
Davies, A, Bentley, M, Field, BS, "Comparison of the Action of Vantocil, Cetrimide, and Chlorhexidine on Escherichia coli and Its Spheroplasts and the Protoplasts of Gram-Positive Bacteria.
Briefly, fungal suspensions were centrifuged for 10 min at 3000g, and 1 mL of lyticase buffer was added to generate spheroplasts [10 kU/L recombinant lyticase (Sigma), 50 mmol/L Tris,1 mmol/L EDTA, 2 mL/L ([beta]-mercaptoethanol].
Spheroplasts were obtained by incubation at 37[grados]C during 45-60 minutes, followed by centrifugation.