sphaeroplast

sphaeroplast

a CELL with a partially degraded cell wall. Compare PROTOPLAST.
References in periodicals archive ?
Sphaeroplast formation in yeast during the transition from exponential phase to stationary phase.
One method to remove cell walls is to use enzymes to break down cell wall components to release the cell's sphaeroplast. This study was undertaken to develop a technique that could provide sphaeroplasts from sporangiospores of the zygomycete, Pilobolus.
However, most techniques describe sphaeroplast formation in basidiomycetes or ascomycetes (Lalithakumari 2000).Little work has been done with zygomycetes and an extensive search of the literature revealed no protocols for removing Pilobolus sporangiospore cell walls.
This inter-spore adhesion is an impediment to sphaeroplast production from Pilobolus sporangiospores.
Keywords: Pilobolus, chitinase, chitosanase, sphaeroplasts, zygomycetes
All treatment solutions contained 1.5 M to 3.2 M sorbitol which reduced cytolysis of sphaeroplasts, and Tween 20[R] to reduce sporangiospore clumping.
Ungerminated sporangiospores were used to produce sphaeroplasts by introducing 8-10 sporangia, collected from a Petri dish lid, to a 1.5 ml micro-centrifuge tube containing 100 [degrees]l of one of the enzyme solution regimens listed above.
Initial studies compared the use of germ tubes of germinating spores, and ungerminated spores, as sources of sphaeroplasts. The germinated spores were exposed to several different enzyme regimens.
All five enzyme treatments of germinated sporangiospores resulted in production of sphaeroplasts. However, microscopic examination of spores treated by all of these techniques showed that most spores had not ruptured.
Treatments with a single enzyme at the enzyme optimum temperatures and pH caused cell lysis with the release of many sphaeroplasts; however, large numbers of intact spores remained.
Microscopic techniques were used to determine whether or not sporangiospore cell walls were being degraded with the release of sphaeroplasts as a result of enzyme treatments.
They have also been used as vaccine [10], antitumor[11], tumor marker[12] and useful biomarker in Gaucher disease[13] and used in the preparation of sphaeroplasts and protoplasts from yeasts and fungal species which can be used further for biotechnological uses[14]..