sphaeroplast

sphaeroplast

a CELL with a partially degraded cell wall. Compare PROTOPLAST.
References in periodicals archive ?
Sphaeroplast formation in yeast during the transition from exponential phase to stationary phase.
One method to remove cell walls is to use enzymes to break down cell wall components to release the cell's sphaeroplast.
However, most techniques describe sphaeroplast formation in basidiomycetes or ascomycetes (Lalithakumari 2000).
Following incubation, 15 [micro]l samples of spore suspension were removed and examined for sphaeroplast formation using brightfield, phase contrast, fluorescent, or Huffman modulated interference contrast microscopy.
Figure 2 shows a single sphaeroplast emerging from its fluorescing spore wall stained with calcofluor white.
This inter-spore adhesion is an impediment to sphaeroplast production from Pilobolus sporangiospores.
Keywords: Pilobolus, chitinase, chitosanase, sphaeroplasts, zygomycetes
2 M sorbitol which reduced cytolysis of sphaeroplasts, and Tween 20[R] to reduce sporangiospore clumping.
Initial studies compared the use of germ tubes of germinating spores, and ungerminated spores, as sources of sphaeroplasts.
All five enzyme treatments of germinated sporangiospores resulted in production of sphaeroplasts.
5 produced a higher proportion of sphaeroplasts than any other regimen of enzymes.
They have also been used as vaccine [10], antitumor[11], tumor marker[12] and useful biomarker in Gaucher disease[13] and used in the preparation of sphaeroplasts and protoplasts from yeasts and fungal species which can be used further for biotechnological uses[14].