Enot et al., "Spermidine
induces autophagy by inhibiting the acetyltransferase EP300," Cell Death and Differentiation, vol.
promotes stress resistance in Drosophila melanogaster through autophagy-dependent and -independent pathways.
The reaction mixture (10 [micro]L) contained 2ng/[micro]L, 1,000 bp DNA template with a T7 promoter, transcription buffer, 2 mM spermidine
, 10 mM DTT, 2 mM of each rNTP, 2 units//Th RNasin, and 6 [micro]L of collected QD: T7RNAP solution.
, a novel polyamine-related compound that accumulates during ovary and petal senescence in pea.
The researchers had shown earlier that polyamines such as spermidine
and spermine enhance nutritional and processing quality of tomato fruits.
is also found in wheatgerm, grapefruit and soya beans.
The main aim of this research was to determine the effects of polyamines (putrescine and spermidine
) on gynogenic ability of two Iranian onion cultivars which one of them (S.
With coverage of both theory and practical aspects, this broad overview considers a range of interactions between DNA and such materials as metal ions, polyelectrolytes, spermine, spermidine
, surfactants and lipids, and proteins.
Efficient plant regeneration from long-term callus cultures of rice by spermidine
. Plant Cell Reports, v.14, n.11, p.717-720, 1995.
Abbreviations: ABA, abscisic acid; ACC, 1-aminocyclopropane-1-carboxylic acid; DAP, days after pollination; IAA, indoleacetic acid; PCA, perchloric acid; PUT, putrescine; SPD, spermidine
; SPM, spermine.
Ten micrograms of genomic DNA was digested with each enzyme (BamHI, BamHI/HindIII, EcoRI, and BgIII; Gibco-BRL, Gaithersburg, Md) in the following reaction mixture: 10 [micro]g of DNA, sterile double-distilled water to 39 [micro]L total, 5 [micro]L of 10x buffer, 4 [micro]L of restriction enzyme (50 units/[micro]L stock), and 2 [micro]L of 0.1 mol/L spermidine
(Sigma Chemical Co, St Louis, Mo).
Total DNA samples were digested according to manufacturer's specifications, with the addition of spermidine
(0.1 mM final concentration) to facilitate completion of enzymatic digestion.