Testosterone promotes the conversion of round spermatids
between stages VII and VIII of the rat spermatogenic cycle.
Scoring 6 is characterized by the development of spermatogenic cells up to early spermatids
and scoring 7 by the development of spermatogenic cells up to late spermatids
The diameters of germinal cell nuclei were calculated by measuring 20 nuclei of each cell type at cross sections of seminiferous tubule stages, with the exception of elongated spermatids
, in which the lengths of nuclei in the cells were measured.
The groups of stages I-VI (one spermatids
generation), VII-VIII (spermatozoa), IXXIII (two generations of spermatids
) and XIV (secondary spermatocyte) of the seminiferous epithelium cycle (Leblond & Clemont, 1952) were identified under a light microscope (Leica DMLS) at x 200 magnification.
In a study treatment with alcoholic extract of Nigella sativa (0.5 and 1.5 g/kg) led to significant increase in body weight gain (g), reproductive parameters (seminiferous tubules thickness and diameters, account of spermatogonia, primary and secondary spermatocytes, spermatids
, free spermatozoa, account of sertoli and Leydig cells, diameter of Leydig cells and the height of epithelial cells entirely covered epididymal caudal), hormones (testosterone and follicle stimulating hormone) as well as protein concentration .
During this process, the germ line stem cells embedded in the supporting Sertoli cells of the seminiferous tubules divide mitotically to expand the pool of spermatogonia; after two meiotic divisions, these actions will give rise to spermatocytes and haploid spermatids
. The spermatids
subsequently undergo a morphological and molecular remodeling process, known as spermiogenesis, during which the head, middle piece, and flagellum of the spermatozoa differentiate (Eddy, 2006).
"Role of non-receptor protein tyrosine kinases in spermatid
transport during spermatogenesis," Seminars in Cell & Developmental Biology 30: 65-74.
While the expression of MARCH7 increases in developing rat spermatids
, the expression of MARCH10 is restricting to elongating and elongated spermatids
but is not expressed in the epididymal spermatozoa, that is, almost fully developed spermatids
Ultrastructural and cytochemical studies of the spermatid
and spermatozoon of Culex quinquefasciatus (Culicidae).
Yasuhiro Yamauchi, a post-doctoral scholar on Ward's team, harvested these immature spermatids
and used a technique called round spermatid
injection (ROSI) to successfully fertilize oocytes in the laboratory.
required for spermatid
elongation and DNA condensation during spermiogenesis.
Number: TA-060-UG) and then Bax- and Bcl-2 positive spermatogenic cells (from spermatogonia to elongated spermatid
) were evaluated under light microscope and scored according to Kandi Coskun and Cobanoglu (2005) as follows: 0, no stained cells; 1, <25% positive-stained cells; 2, 26-50% positive-stained cells; 3, 51-75% positive-stained cells; and 4, >75% positive-stained cells.