Evaluation of human sperm hyperactivated motility and its relationship with the zona-free hamster oocyte sperm penetration
MMP2 and acrosin are major proteinases associated with the inner acrosomal membrane and may cooperate in sperm penetration
of the zona pellucida during fertilization.
showed the relationship of current use of cigarettes and alcohol to the parameters of seminal fluid analysis, sperm penetration
assay, and sperm autoimmunity.
This failure of the IVC systems results in a poor release of cortical granules after sperm penetration
, thus resulting in failure of the zona pellucida reaction, and consequently in polyspermy (FUNAHASHI et al., 2000).
of Vitelline Envelope of SicyoniaIngentis Eggs is Mediated by a Trypsin like Lysin of Acrosomal Vesicle Origin.
The results of zona-free hamster oocyte sperm penetration
(SPA) assay, which examines the ability of spermatozoa for capacitation and used for prediction of the likelihood of spontaneous pregnancy in vivo as well as successful fertilization using IVF, are not considered meaningful because of the false positive and false negative rates (Consensus Workshop on Advanced Diagnostic Andrology Techniques, 1996).
(3,5,6) Furthermore, recent hydrodynamic estimation of the flagellar force calculated by the resistive-force theory using the real hyperactivated flagellar waves disclosed an important role of low beat frequency of the hyperactivated flagellar movement; namely, the slowly oscillating lateral force generated by the hyperactivated flagellar movement is most effective for sperm penetration
through the zona pellucida.
The Global Male Infertility market can be divided into seven segments: DNA Fragmentation Technique, Oxidative Stress Analysis, Microscopic Examination, Sperm Penetration
Assay, Sperm Agglutination, CASA, and Others.
The results of tests using Acrobeads show good reproducibility and are correlated with the results of sperm penetration
assays using zona-free hamster eggs and IVF [24, 33, 34].
The duration of sperm motility was much lower than the average time of opening of the micropyle, 27 seconds, and in this way it can reduce the sperm penetration
On the one hand, there is selection on females to slow down sperm penetration
to allow the establishment of an effective block against further sperm penetration
Evaluation of sperm penetration
of oocytes in vitro