The specific absorbance
of SOF in each medium was calculated as shown in Table-1.
The absorbance spectrum of [I.sub.3.sup.-] in acetonitrile has specific absorbance
peaks at around 290nm and 360nm .
If dissolved in ethanol it presents a purple color with a specific absorbance
at 517 nm.
The wavelengths were chosen to correspond to the specific absorbance
characteristics of the hemoglobin derivatives (Fig.
Table 2 shows the specific absorbance change for each assay.
The specific absorbance change for the tPA/PAI-1 calibrator was evaluated as a measure of assay response.