In addition to dimerization of FokI nuclease domains, ZFN requires correct spacer sequence
(5-6 bp) and orientation of chimeric nucleases for the cleavage of dsDNA [22, 23].
In order to enhance acquisition of spacer sequences
flanked by non-NGG PAM motifs, SpCas9 variants were created by error-prone PCR, and after selection, a Cas9 variant I473F was identified with higher specificity for the PAM sequence 5'-NAG-3' .
Longibrachiatum including related teleomorphs based on analysis of ribosomal DNA internal transcribed spacer sequences
. Mycologia, 1997; 89: 442-460.
Floeter-Winter, "The Trypanosoma cruzi ribosomal RNA-encoding gene: analysis of promoter and upstream intergenic spacer sequences
," Gene, vol.
baumannii complex by restriction analysis of the 16S-23S rRNA intergenic spacer sequences
. Journal of Clinical Microbiology.
The team's analysis of CRISPR repeat and spacer sequences
revealed that although there is a set of CRISPRs maintained within each subject over time, ranging from 7 percent to 22 percent, there was a remarkable amount of change observed even in short periods.
Analysis of ribosomal DNA internal transcribed spacer sequences
of Leishmania donovani complex.
Phylogeny and systematic of 18 Colletotrichum species based on ribosomal DNA spacer sequences
. Genome, v.39, n.3, p.499-512, 1996.
Therefore the internal transcribed spacers (ITS) regions are rather interesting for studies on taxonomy and identification, because the spacer sequences
have revealed intraspecific variation when analysed by RFLP, frequently both in sequence and size (Sollner-Webb and Mougey, 1991; Matioli, 2001).