Limited Tenders are invited for Digital sonifier
La disrupcion de los parasitos fue logrado usando un sonicador (Brason sonifier
modelo 150, EUA) a una frecuencia de 7 Hertz y 5 ciclos por 20 seg.
In this case, the cell suspension obtained was then subjected to 3 cycles of repeated freezing and thawing, followed by a 5 min sonication using a Branson Sonifier
250 (VWR Scientific, San Diego, CA).
The suspension was dispersed with a sonication horn (digital Sonifier
450) to break any aggregates of nanoparticles.
After storage at -20[degrees]C, the sample was homogenized in sonicator (Branson Sonifier
Model 250) (3 cycles of 4s each) and the homogenate centrifuged at 2500rpm, 4[degrees]C for 10min.
Triton X-100 was supplemented to a 2% final concentration and sonicated two times using the Branson Sonifier
250 at 30% duty cycle and an output level of 3 for 1 min.
These solutions were shaken with a Branson 2510 sonifier
for 220 min in SET DEGAS and homogenized with a Fisher Vortex Genie 2[TM] shaker at "speed eight" for 10 min (Tavares et al.
A continuacion se formo la emulsion adicionando lentamente la fase organica a la acuosa mientras se realizaba ultrasonido (Branson digital sonifier
All aliquots were centrifuged for 2 min at 1000 rpm, resuspended in PBS, and lysed using a Branson Digital Sonifier
450 (Danbury, CT).
The residue was dissolved in 100 [micro]L methanol, stirred for 1 min, and sonicated for 30 s in a bath sonifier
Finally, PEG coated particles were dispersed in water and the resulting suspension was sonicated for 10 min at room temperature and 100% of the ultrasonic power (Transsonic Digital Bath sonifier
, Elma, EU).
Fish were then homogenized for 10s using an ultrasonic homogenizer (Branson Sonifier