Cells were collected by centrifugation, resuspended in the sonicating
buffer (140 mM NaCl, 2.
Their findings show that thermal sonicating
technology causes weakening of the cell by forming cavitation in the cellular structure and disruption and breakdown of cellular materials, formation of pores, lack or clumping of cellular material, breakdown of cell membrane, and inactivation of the target microorganism.
The powders were carefully washed twice using chloroform, twice by acetone and once by diethyl ether, sonicating
for 10 min every time that fresh solvent was added.
The samples were then removed from the solution and cleaned by sonicating
for 30 minutes in methanol to remove the excess monomer.
We formed suspensions in water by adding dry beads to water, shaking vigorously for 40 s, and then sonicating
for 60 s.
External contamination was removed by sonicating
the nails with 1% Triton X-100 solution for 20 min (Sigma-Aldrich Inc.
44) Graphite nanosheets have been prepared by sonicating
expanded graphite in aqueous alcohol solution.
Total soluble sugars (SS) were determined according to the method of Hall and Haczkaylo (1963) by mixing 1 g ground sample with 40 ml 80% ethanol while sonicating
the mixture for 1 h in a conical flask.
The particular sonication variables (time and amplitude setting) used for removing maternal cells described above were established by sonicating
sperm storage organs for varying lengths of time and for different amplitude settings.
74 g samples of wood flour and extracting this material in 25 mL of methanol in screw cap bottles while sonicating
for 3 hours.
The particle suspensions were used immediately after dilution and sonicated by means of a sonicating
tip with a power output between 7 and 10 W at 22.
The technique consists of cross-linking proteins with DNA in cells on Petri dishes, harvesting the cells, and sonicating
the cells, first to isolate and break down the nuclei and then to fragment the DNA (desired size usually 200-1000 nucleotides).