Reproducible results were obtained by sonicating
a known weight of sample in 20ml of distilled water in a sonic bath for five minutes before adding TISAB and analyzing.
The authors extracted endotoxin from the GF and PC filters by placing each filter in a vial containing 5 mL of sterile, pyrogen-free water (at 37-40[degrees]C) for 60 minutes and sonicating
each vial at 10-minute intervals.
Vortexing swabs for 2 min during processing resulted in superior extraction of spores when compared to sonicating
them for 12 min or subjecting them to minimal agitation.
The flask continued stirring to emulsify for 60 min after which a sonicating
probe (Fisher 300W Sonic Dismembrator used at 70% output [maximum output of 300W] for durations of 25 min) was immersed into this pre-emulsion.
Additional mixing was effected by sonicating
the mixtures in an ultrasound bath for 10 min.
They were also sonicated in a sonicating
water bath for 1minute.
Each day thereafter, inoculated plants were moved to a mist chamber (a saturated atmosphere was maintained by a cool-air, sonicating
humidifier) during daylight hours (minimum of 10 h light) and then were moved back into the dew chamber at night.
2 mL) was added to the microcentrifuge tube containing the scale cover, then the tube was closed and placed in a sonicating
bath for 12 h.
25 M sucrose and 10 mM dithioerythrietol) by sonicating
for 30 sec at 50 watts.
the extract or treating it with detergents did not alleviate the smearing problem.
Dintzis, who casually suggested, "Why not try sonicating
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