In 100 ml measuring flask 50 mg of Orphenadrine citrate was transferred, added 80 ml mobile phase, sonicated
to dissolve and again mobile phase was added to made volume up to the mark.
After centrifugation, cell pellet samples were lysed and sonicated
in a lysis buffer consisting of 10 mM Tris-HCI (pH 7.
Lane M: prestained protein molecular marker (Thermo Scientific Pierce Prestained Protein MW Marker); Lane 1: sonicated
BL21 (DE3) crude cell lysate harboring recombinant plasmid after induction with IPTG; lanes 7 and 8: rPR protein purified by Ni2+-NTA resin column affinity chromatography; Lanes 2-6: E.
The polymer was then re-suspended in 500 ml 5% v/v acetic acid and sonicated
for 10 minutes.
The oxidized CNT sponge thus produced was sonicated
with ethylene diamine and DCC for 1 hour.
The salt mobile phase was sonicated
at 22[degrees]C under vacuum for 5 min before use.
Each tissue sample was sonicated
(Mi-croson XL 2005, Heat System Inc.
Each solution of BCP and CNT was firstly sonicated
for approximately 30min to avoid agglomerates.
SOBM powder material was sonicated
in an ultrasonic bath for 6 hrs with 58 [gmol.
Monkey brain and liver and tick pools were sonicated
in 600 mL of Minimum Essential Media (GIBCO/ BRL, Life Technologies, Grand Island, NY, USA), and 400 mL of media was added to the homogenate.
5 ml of decontaminated AFB smear-positive sputum, TA or BAL specimen was centrifuged, heat inactivated, sonicated
and re- centrifuged.
The plates were placed on an orbital shaker (setting 750 rpm) for 30 min, and sonicated
for 15 min.