burgdorferi using a commercially available enzyme immunoassay (EIA) that used a whole-cell sonicate
The kits guide researchers through a process in which they cross-link and lyse cells, sonicate
chromatin and hybridize biotinylated capture oligos, which bind to the complementary RNA sequence.
Accurately weigh 250 mg of powered GQDG and dissolve it in 50 ml of methanol; sonicate
twice for 30 min each with a short interval; dilute the extracted solution five times with methanol; then, filter through a 0.
(effectively broken, unfractionated cells) induced MMP-1 ('interstitial collagenase') and MMP-9 messenger RNAs, and the expression of MMP-9, but not of MMP-1 was dependent on Toll-like receptor-2.
If the preparation of the acryloid solution produces many air bubbles (as a consequence of stirring the pellets in acetone), sonicate
the vial until all the bubbles have risen to the surface.
burgdorferi by using a whole-cell sonicate
ELISA, C6 ELISA, or Western blot assay as described (16,20-22).
Serological testes used for the detection of CT crosss-reactive material in the culture filtrates were (i) ganglioside GM1 ELISA in which ELISA plate coated with GM1 ganglioside was reacted with culture supernatant and rabbit polyclonal CT antibody, (ii) direct ELISA in which ELISA plate was directly coated with bacterial sonicate
and reacted with CT antibody, and (iii) immunoblot assay in which bacteria-free culture filtrate, crude outer membrane protein (OMP), or sarcosyl-purified OMP was separated by SDS-PAGE, transferred to nitrocellulose membrane and probed with rabbit anti-CT antibody.
0 25 Detection wavelength 205 nm Run time 45 min Sample preparation Weigh accurately 500 mg extract to a 100 ml volumetric flask dissolve in 50 ml methanol, sonicate
for 10-15 min.
Extraction Extraction First water Sample type solvent (a) method addition Air filter 5 mL x 2 Sonicate
100 mL Air PUF 30 mL x 4 Squeeze 80 mL Impactor plate 5 mL x 2 Sonicate
100 mL Impactor filter 10 mL x 2 Sonicate
100 mL Surface wipe 20 mL x 2 Sonicate
360 mL PUF roller sleeve 150 mL x 4 Squeeze 150 mL Floor dust (bulk) 25 mL Sonicate
100 mL Hexane Second water Sample type partition addition Air filter 20 mL x 2 100 mL Air PUF 20 mL x 2 70 mL Impactor plate 20 mL x 2 100 mL Impactor filter 20 mL x 2 100 mL Surface wipe 20 mL x 2 0 mL PUF roller sleeve 25 mL x 2 0 mL Floor dust (bulk) 20 mL x 2 80 mL Variation in standard procedure, scaled to size of sample matrix.
Th2-type immune response observed in healthy individuals to sonicate
antigen prepared from the most prevalent Mycobacterium tuberculosis strain with single copy of IS6110.
9%) to dissolve and sonicate
for about 5 min, dilute to volume with methanol and mix.
Samples obtained from these patients were tested by a commercial enzyme-linked immunosorbent assay (ELISA) (PanBio, East Brisbane, Queensland, Australia) for the presence of immunoglobulin (Ig) A against a Bordetella pertussis sonicate
and by an in-house IgA-detection ELISA directed against pertussis toxin as previously described (9).