WCS C6 peptide IgM/IgG microti Patient EIA EIA immunoblot (IFA) * A neg neg neg <1:8 B neg neg neg <1:8 C neg neg neg <1:8 D neg neg neg <1:8 E neg neg neg <1:8 F neg neg neg 1:64 G neg neg neg 1:64 H neg neg neg <1:8 I neg neg neg <1:8 J neg neg neg <1:8 K neg neg neg <1:8 L neg neg neg <1:8 M neg pos neg <1:8 N neg neg neg <1:8 Abbreviations: EIA = enzyme immunoassay; F = female; IFA = indirect fluorescent antibody; Ig = immunoglobulin; IgG = immunoglobulin G; IgM = immunoglobulin M; M = male; neg = negative; NP = not provided; pos = positive; WCS = whole cell sonicate
. * For patients A-E, the specimens tested by IFA were collected a median of 282 days (range = 30-323 days) after the specimens tested by EIA.
However, this was also observed for a sonicate
of the mixture of the bacterial host used for propagation of the phages, which are components of MS-1.
proposed a 2-tiered EIA approach consisting of 2 FDA-cleared EIAs: whole-cell sonicate
EIA followed by reflex C6 EIA.
Further studies are required to quantify the number of microorganisms in sonicate
fluid and assess the boundaries between PJI and contamination of explanted prosthesis following aseptic loosening .
Purification of fusion GST-SK Total recovery Steps Protein SK Specific Fold % (mg) (IU) activity purified Yield Initial 12.7 120500 9488 -- -- (Sonicate
) Elution 4.25 113270 26652 2.8 94 (Purified) Discussion
leprae whole cell sonicate
or PPD (purified protein derivative of M.
Accurately weigh 250 mg of powered GQDG and dissolve it in 50 ml of methanol; sonicate
twice for 30 min each with a short interval; dilute the extracted solution five times with methanol; then, filter through a 0.45[mu] filter membrane; use the filtrate as sample solution.
Serological response (Western blot) to fractions of Mycobacterium tuberculosis sonicate
antigen in tuberculosis patients and contacts.
acnes (as a 'sonicate
') on human monocytes in culture in affecting the production and activities of the matrix metalloproteinases (MMPs), of which there are over 20 with different specificities that may be responsible for tissue destruction and, potentially, scarring.
If the preparation of the acryloid solution produces many air bubbles (as a consequence of stirring the pellets in acetone), sonicate
the vial until all the bubbles have risen to the surface.
For this fractionation scheme, soils (1 g) were sequentially extracted via shaking with 30mL of deionised water (shake 2 h), 0.5 M NaHC[O.sub.3] (shake 16h), 0.1 M NaOH (shake 16h), 0.5 M [H.sub.2]S[O.sub.4] (shake 16h), and finally 0.1 M NaOH (sonicate
for 5 min then shake 16 h), before the remaining P is removed by persulfate digestion (0.3 g [K.sub.2][S.sub.2][O.sub.8] in 2 mL 0.5 M [H.sub.2]S[O.sub.4], 150[degrees]C for 2h).
Typical workstation capabilities include moving, stacking, unstacking, coveting and uncovering plates; pipetting with fixed or disposable tips and variable spacing of the pipettors; mixing (vortex or sonicate
); incubation (heat or cool); and load/unload plates onto ancillary plate readers and washers.