The medium employed for growth and enzyme production consisted of (%): 0.1 K[H.sub.2]P[O.sub.4], 0.25 [Na.sub.2]HP[O.sub.4], 0.1 NaCl, 0.2 [(N[H.sub.4]).sub.2]S[O.sub.4], 0.005 MgS[O.sub.4].7[H.sub.2]O, 0.005 Ca[Cl.sub.2], 0.2 tryptone, and 1% soluble starch
Enzyme [Q.sub.10] (20-35 [degrees] C range) Sucrose synthase 1.90 UDP glucose pyrophosphorylase 1.52 Glucokinase 1.53 ATP-linked fructokinase 2.62 UTP-linked fructokinase 1.41 Phosphoglucomutase 1.62 Phosphoglucoisomerase 2.35 ATP-linked phosphofructokinase 1.33 PPi-linked phosphofructokinase 0.97 ADP glucose pyrophosphorylase 1.32 Soluble starch
synthase 1.33 RESULTS AND DISCUSSION
The solid dispersions of florfenicol and diclazuril have first been prepared with PEG6000 as carriers, and then the soluble powder was made with the two solid dispersions, soluble starch
, and glucose.
synthases (SSS), starch branching enzymes (SBE) were also responsible for starch biosynthesis and controlling its chain length fine structure of amylopectin (Mizuno et al., 2001; Yandeau- Nelson et al., 2011).
In table 9,10,11 and 12 shows the opacity and pH value of medium which contain different concentration of Soluble starch
. It showed low activity in the low concentration of soluble starch
Studies on substrate specificity of the enzyme from both the crude extract and post dialysis solution upon three different substrates: Soluble starch
, glycogen and dextrin the result was showed in graph1.
Quantification of total protein, resistant and soluble starch
, sucrose, glucose and fructose: these analyses were done using 50 mg of EC and NEC of mahogany.
Starch-agar plate assay: Soluble starch
(1 gm) and agar (2 gm) was mixed in 100 ml of distilled water and poured on a disposable plate.
A volume of 0.5 ml of the substrate (1% of soluble starch
in the acetate buffer) at 50[degrees]C was added into the test tube containing 0.5 ml of the enzyme (fermentation broth) for 30 minutes.
For this procedure, 3 mL of the starch solution was placed in a test tube and 1 mL of an I / KI solution was added; then the intensity of the blue color produced in a 600 nm spectrophotometer was measured against a reagent blank.; the amount of starch present in the sample was calculated from a standard curve prepared in the range of 1050 mg of soluble starch
/ mL, treated in the same manner as the test sample.
In current research, a non-linear mixed-effects model is investigated to determine the impact of acarbose on postprandial glycemia in a single rat after the ingestion of soluble starch
. The analysis was carried out with R Statistical Software (R Core Team, 2015) using NLME package (Pinheiro, Bates, Debroy & Sarkar, 2007).
Medium contained soluble starch
20 g/L, peptone 20 g/L, and agar 20 g/L, then culture was spread, and plates were incubated for 48 h.