LC3 and p62 mRNA expressions in
soleus muscles of wistar rats
Regarding the
soleus muscle, aerobic training in the aquatic environment (ATS) was responsible for generating greater hypertrophy than treadmill training (ATT).
Time-course of body mass (A), changes of body mass (B),
soleus muscle mass (C), extensor digitorum longus (EDL) muscle mass (D), EDL fiber cross-sectional area (CSA) and corresponding microphotograph (E), rotarod test performance pre- and post-RET protocol (F), and ambulation test performance pre- and post-RET protocol (G) for control, fasting and fasting + RET experimental groups.
The HRT of diabetic
soleus muscle (table-II) was significantly prolonged while that of EDL was similar to the healthy controls (table-III).
The EDL and
soleus muscles had no significant differences in specific force (s[P.sub.O]) production, fatigability, and recovery from fatigue between the mdx:[Seps1.sup.-/+] mice and mdx littermate controls (Figure 4 and Supplementary Figure 1, resp.).
To further evaluate HU-induced atrophy, frozen sections of
soleus muscle from the control and unloaded mice were immunostained with antilaminin (Figure 1(e)).
To collect the
soleus muscle, mice were sacrificed by decapitation 20 min after i.p.
Total RNA was extracted from
soleus muscles with TRIzol Reagent (Invitrogen Life Technologies, Carlsbad, CA, USA) and treated with DNase I (Invitrogen Life Technologies).
Results showed that there was a positive correlation between plasma nitrite and capillary density around in
soleus muscle (R2 = 0.65), while, there was a weak positive correlation between plasma nitrite and capillary density in FHL muscle ([R.sup.2] = 0.0039) (Figure 5).
Like most of the anatomical variations of the human muscular system, the accessory
soleus muscle was well known to early anatomists as well.
The gastrocllemius muscles had the weight at 58.0 [+ or -] 3.1% (n = 8) of the contralateral control value, and the
soleus muscles were 52.4 [+ or -] 5.0% (n = 8) of the control value.
According to previous reports, a 30-min bout of passive stretching induces the expression of myogenic differentiation factor in
soleus muscles [4].