slice culture


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slice culture

A means of studying living tissues by obtaining specimens from approx.100 to 400 μm in thickness and maintaining them in vitro in a nutrient bath. The technique is used in investigations of brain or liver diseases.
See also: culture
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The physiological relevance of these observations was confirmed using hippocampal slice cultures from PD5 rats biolistically labeled with TFP at 3 DIV and then exposed to PCBs from 5-7 DIV.
Confocal imaging of microglial cell dynamics in hippocampal slice cultures.
At 3 DIV, slice cultures were biolistically transfected with plasmid-encoding tomato fluorescent protein (TFP) using the Helios gene gun (Bio-Rad, Hercules, CA) per the manufacturer's directions.