Gebremedhin et al., "Protective effect of 20-HETE inhibition in a model of oxygen-glucose deprivation in hippocampal slice cultures
," American Journal of Physiology--Heart and Circulatory Physiology, vol.
Caption: Figure 3: RA-NP modulated microglia activation and morphology in LPS- treated hippocampal slice cultures. Murine organotypic hippocampal slice cultures (P7) were treated with RA-NP (10 [micro]g/mL) or free RA (0.4 [micro]M), and their effect on cell morphology was quantified in an inflammatory context (100 ng/mL LPS, 24 hours).
(b) Representative images depicting cell death on organotypic hippocampal slice cultures. Slices were counterstained with propidium iodide (PI).
Following treatments, slice cultures were transferred to a temperature-controlled chamber (30[degrees]C) mounted on an upright confocal microscope equipped with W Plan-APOCHROMAT 63x/1.0 objective (Zeiss LSM710, Carl Zeiss MicroImaging GmbH) and continuously perfused with Tyrode solution containing (in mM): NaCl, 137; KCl, 2.7; Ca[Cl.sub.2], 2.5; Mg[Cl.sub.2], 2; NaHC[O.sub.3], 11.6; Na[H.sub.2] P[O.sub.4], 0.4; and glucose, 5.6 (pH 7.4).
Following treatment, hippocampal slice cultures were removed from the cover glass and fixed in 4% paraformaldehyde dissolved in 0.1 M phosphate buffer (PB) overnight at 4[degrees]C.
The physiological relevance of these observations was confirmed using hippocampal slice cultures from PD5 rats biolistically labeled with TFP at 3 DIV and then exposed to PCBs from 5-7 DIV.
Acute hippocampal slice preparation and hippocampal slice cultures. Methods Mol Biol 758:115-134.
The Plasma Membrane [Ca.sup.2+] -ATPase (PMCA2) Is Strongly Expressed in Purkinje Cell Dendrites in Cerebellar Slice Cultures
. T- and P/Q-type [Ca.sup.2+] channels are abundantly expressed in Purkinje cell dendrites [18, 19] and are one of the major sources of [Ca.sup.2+] influx into Purkinje cells [18, 20-22].
In support of this hypothesis, we observed that the selective RyR antagonist, FLA365, as well as siRNA knockdown of either RyR1 or RyR2, completely blocked BIC-induced dendritic growth in both dissociated cultures of hippocampal neurons and hippocampal slice cultures
Cytotoxicity in slice cultures
was assayed by lactate dehydrogenase (LDH) release to media and by cellular uptake of propidium iodide (PI) (Jourdi et al.