skin biopsy

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Skin Biopsy



A skin biopsy is a procedure in which a small piece of living skin is removed from the body for examination, usually under a microscope, to establish a precise diagnosis. Skin biopsies are usually brief, straightforward procedures performed by a skin specialist (dermatologist) or family physician.


The word biopsy is taken from Greek words that mean "to view life." The term describes what a specialist in identifying diseases (pathologist) does with tissue obtained from a skin biopsy. The pathologist visually examines the tissue under a microscope.
A skin biopsy is used to make a diagnosis of many skin disorders. Information from the biopsy also helps the doctor choose the best treatment for the patient.
Doctors perform skin biopsies to:
  • make a diagnosis
  • confirm a diagnosis made from the patient's medical history and a physical examination
  • check whether a treatment prescribed for a previously diagnosed condition is working
  • check the edges of tissue removed with a tumor to make certain it contains all the diseased tissue
Skin biopsies also can serve a therapeutic purpose. Many skin abnormalities (lesions) can be removed completely during the biopsy procedure.


A patient taking aspirin or another blood thinner (anticoagulant) may be asked to stop taking them a week or more before the skin biopsy. This adjustment in medication will prevent excessive bleeding during the procedure and allow for normal blood clotting.
Some patients are allergic to lidocaine, the numbing agent most frequently used during a skin biopsy. The doctor can usually substitute another anesthetic agent.


The first part of the skin biopsy test is obtaining a sample of tissue that best represents the lesion being evaluated. Many biopsy techniques are available. The choice of technique and precise location from which to take the biopsy material are determined by factors such as the type and shape of the lesion. Biopsies can be classified as excisional or incisional. In excisional biopsy, the lesion is completely removed; in incisional biopsy, a portion of the lesion is removed.
The most common biopsy techniques are:
  • Shave biopsy. A scalpel or razor blade is used to shave off a thin layer of the lesion parallel to the skin.
  • Punch biopsy. A small cylindrical punch is screwed into the lesion through the full thickness of the skin and a plug of tissue is removed. A stitch or two may be needed to close the wound.
  • Scalpel biopsy. A scalpel is used to make a standard surgical incision or excision to remove tissue. This technique is most often used for large or deep lesions. The wound is closed with stitches.
  • Scissors biopsy. Scissors are used to snip off surface (superficial) skin growths and lesions that grow from a stem or column of tissue. Such growths are sometimes seen on the eyelids or neck.
After the biopsy tissue is removed, bleeding may be controlled by applying pressure or by burning with electricity or chemicals. Antibiotics often are applied to the wound to prevent infection. Stitches may be placed in the wound, or the wound may be bandaged and allowed to heal on its own.
The second part of the skin biopsy test is handling and examining the tissue sample. Drying and structural damage to the tissue sample must be prevented, so it should be placed immediately in an appropriate preservative, such as formaldehyde.
The pathologist can use a variety of laboratory techniques to process the biopsy tissue. Tissue stains and several different kinds of microscopes are used. Because there are many skin disorders (broadly called dermatosis and dermatitis), the pathologist has extensive training in their accurate identification. Cases of melanoma, the most malignant kind of skin cancer, have almost tripled in the past 30 years. Because melanoma grows very rapidly in the skin, quick and accurate diagnosis is important.


The area of the biopsy is cleansed thoroughly with alcohol or a disinfectant containing iodine. Sterile cloths (drapes) may be positioned, and a local anesthetic, usually lidocaine, is injected into the skin near the lesion. Sometimes the anesthetic contains epinephrine, a drug that helps reduce bleeding during the biopsy. Sterile gloves and surgical instruments are always used to reduce the risk of infection.


If stitches have been placed, they should be kept clean and dry until removed. Stitches are usually removed five to 10 days after the biopsy. Sometimes the patient is instructed to put protective ointment on the stitches before showering. Wounds that have not been stitched should be cleaned with soap and water daily until they heal. Adhesive strips should be left in place for two to three weeks. Pain medications usually are not necessary.


Infection and bleeding occur rarely after skin biopsy. If the skin biopsy may leave a scar, the patient usually is asked to give informed consent before the test.

Normal results

The biopsy reveals normal skin layers.

Key terms

Benign — Noncancerous.
Dermatitis — A skin disorder that causes inflammation, that is, redness, swelling, heat, and pain.
Dermatologist — A doctor who specializes in skin care and treatment.
Dermatosis — A noninflammatory skin disorder.
Lesion — An area of abnormal or injured skin.
Malignant — Cancerous.
Pathologist — A person who specializes in studying diseases. In particular, this person examines the structural and functional changes in the tissues and organs of the body that are caused by disease or that cause disease themselves.

Abnormal results

The biopsy reveals a noncancerous (benign) or cancerous (malignant) lesion. Benign lesions may require treatment.



American Academy of Dermatology. 930 N. Meacham Road, P.O. Box 4014, Schaumburg, IL 60168-4014. (847) 330-0230. Fax: (847) 330-0050.
Gale Encyclopedia of Medicine. Copyright 2008 The Gale Group, Inc. All rights reserved.

skin biopsy

A portion of diseased skin removed for laboratory analysis, usually under local anaesthesia.
Collins Dictionary of Medicine © Robert M. Youngson 2004, 2005
References in periodicals archive ?
* Correlation of C3 fluorescence in Tzanck smears as compared to skin biopsy.
Table 3 Types and respective indications of skin biopsy [7,8].
Caption: Figure 5: Skin biopsy: viral cytoplasmic effect including multinucleated cells and marginalization of chromatin.
Caption: Figure 2: Hematoxylin and eosin stained skin biopsy showing mixed dermal infiltrate consisting predominantly of neutrophils (red arrow), admixed with eosinophils (yellow arrow), and lymphocytes (blue arrow), magnification = 40 x.
Skin biopsy is frequently reported as consistent with leukocytoclastic vasculitis.
Evaluation of real-time and conventional PCR targeting complex 85 genes for detection of Mycobacterium leprae DNA in skin biopsy samples from patients diagnosed with leprosy.
A skin biopsy of the right leg showed deep subcutaneous and fascial inflammation, both perivascular and panniculitis, with nodular lymphocytic infiltrates, many plasma cells, a few eosinophils, and a few granulomas.
A skin biopsy may be required to support the diagnosis.
Clinical information provided on the skin biopsy requisition is often lacking or suboptimal.
All patients who have already received treatment for leprosy, patients with pure neural leprosy, patient not giving their consent for skin biopsy and patients with lepra reactions were excluded from this study.
The skin biopsy in both the cases showed non-caseating perivascular and periappendageal granulomas composed of epithelioid cells and lymphocytes in the superficial and mid-dermis [Figure 2(a)].