High-efficiency FLP and [PHI]C31 site-specific recombination
in mammalian cells.
Johnston, IA) has patented methods of controlling gene silencing using site-specific recombination
Remaining chapters discuss the use of homologous recombination and site-specific recombination
to disrupt or modify endogenous genes and genomes; strategies for controlling transgene expression; position effects, dosage effects, genetic background, transgene structure, and other aspects of transgene behavior that can generate unexpected results in gene transfer experiments; the use of gene transfer to achieve the targeted or random inactivation of endogenous genes; and the major application of gene transfer, including commercial synthesis of recombinant proteins, domestic animal improvement, disease modeling, and gene therapy.
Integrons are efficient gene-capture systems by site-specific recombination
and are involved in antimicrobial-drug resistance in gram-negative bacteria (2).
The Cre-lox technology involves site-specific recombination
of DNA using Cre recombinase and lox DNA sites.
The Rockefeller University (New York, NY) has patented an inducible promoter system in conjunction with a site-specific recombination
system which allows (i) specific activation of transgenes at specific times or (ii) excision and removal of transgenes (e.
That SGI1 has the same chromosomal location in the different serovars suggests that its insertion occured through site-specific recombination.
Integration of the element has been experimentally shown to occur through site-specific recombination in a 17-bp sequence found in the circular form of the SXT element and a similar 17-bp sequence of prfC of the V.
Based on the well- characterized phage lambda site-specific recombination system, Gateway Cloning Technology provides a highly efficient alternative to the two current conventional cloning and expression methods--restriction enzyme digestion and the cloning of PCR products.
Gateway Cloning Technology replaces restriction enzyme digestion, ligations, gel electrophoresis and analysis, and additional PCR amplification, with the fidelity and efficiency of site-specific recombination.
Integrons: novel DNA elements which capture genes by site-specific recombination
This is the target for site-specific recombination
involved in the insertion and excision of gene cassettes (4,5,15).