In order to analyze the most common PTGS2 haplotypes, different PR and 3'-UTR variants were generated by site-directed mutagenesis
using overlap extension PCR (14).
In general, this has the advantage of being inexpensive and technically easy, since site-directed mutagenesis
techniques are well-developed.
Caption: University of British Columbia biochemist and businessman Michael Smith shared the 1993 Nobel Prize in Chemistry with American Kary Mullis for their work developing site-directed mutagenesis
For the site-directed mutagenesis
assay of the PpPbp gene, we constructed four pTFH22.
Protein engineering approach based on combined use of error-prone PCR mutagenesis, molecular modeling, site saturation and site-directed mutagenesis
(17) were used to enhance the catalytic efficiency of CPC acylase.
Examples of projects that we have tried include screening yeast-two hybrid libraries, performing quantitative PCR, site-directed mutagenesis
, and cloning; see Table 1).
These studies can not be accomplished using standard site-directed mutagenesis
protocols and further characterization due to variety activities that this protein displays, the novelty of its discovery and the absence of a solved crystal structure.
Gly69 variants were generated using site-directed mutagenesis
(Beyotime Biotechnology, China) with the use of wild-type (WT) human DMP1 (containing p.
(SDM) is a technique to create cloned DNAs with modified sequences and the purpose behind this modification is to examine the significance of specific residues in protein structure and function.
The company added that the QuikChange HT Protein Engineering System is a site-directed mutagenesis
solution that creates libraries of rationally designed protein variants.
The product is a site-directed mutagenesis
solution creating libraries of rationally designed protein variants, whose screening enables researchers to gain insights into protein structure and its effects on function within 24 hours, while searching for enhanced forms, all in one experiment.
In other interesting cases, homology models combined with electrophysiology and site-directed mutagenesis
experiments were used to characterize the open conformation and accessibilities of an important variety of voltage-gated ion channels, characterizing their different activation states [13-15].