Assessment of the pharmacological effect of silymarin on ethanol-induced DNA damage by single-cell gel electrophoresis
Alkaline single-cell gel electrophoresis (comet assay): a simple technique to show genomic instability in sporadic breast cancer.
Single-cell gel electrophoresis (comet assay): methodology, potential applications, and limitations in cancer research.
They examined the genotoxicity of silica, titanium dioxide, polystyrine and C60 fullerene nanoparticle suspensions using the alkaline single-cell gel electrophoresis assay (Comet assay) to quantify breaks in single and double stranded DNA.
DNA damage products have been identified and quantified by a wide range of analytical techniques, such as single-cell gel electrophoresis, '2P- postlabeling, immunoassay, gas chromatography/mass spectrometry, high performance liquid chromatography, and electrochemical and electrochemiluminescence sensors [7-9].
The in vitro genotoxicity of imazalil and thiabendazole fungicides and the insecticide chlorpyrifos, compounds used in Costa Rican banana plantations, was evaluated with the single-cell gel electrophoresis technique (comet assay).
Key words: Single-cell gel electrophoresis, comet assay, imazalil, chlorpyrifos, thiabendazole, lymphocytes, genotoxic.
We observed that DMSO and CAT basically abolished most of the As-induced DNA single strand breaks (SSBs) in human cells with single-cell gel electrophoresis
(SCGE) assay (5).