The mutation 2466 C [right arrow] T was a silent mutation
. Mutation 1442 T [right arrow] C leads to the amino acid substitution Val 159 [right arrow] Ala, located in the variable region close to the glycosylation site.
The five W-Beijing isolates with a mutation in mutT4 and a wild-type mutT2 gene did not contain the ogt silent mutation
on codon 12 either.
Additionally, rare inactivating variants (TPMT*3B, *3D, *4, *5, *6, *7, and *8) as well as some intronic and silent mutations
have been reported, although mostly in single individuals (16, 24, 27) (Fig.
are often described in fluoroquinolone sensitive and resistant strains and many combinations of transitions and mutations may exist.
Mutations in codon 503 520 and 554 were silent mutations
as they did not change the encoded amino acid.
DNA sequencing of direct patient samples (or if not available, isolates) with possible RMP resistance should include genetic loci associated with resistance to RMP (to include rpoB) as well as isoniazid (to include inhA and katG) to assess for multidrug/ resistant tuberculosis; rpoB mutations detected by the Xpert MTB/RIF assay might be silent mutations
that do not affect RMP susceptibility.
To compare results generated from gDNA and WGA samples, we analyzed all detected variants, including SNPs, silent mutations
, and variants located in introns or untranslated regions.
Three silent mutations
in other sheep breeds, except for Minxian Black-fur and Kazakh Fat-Rumped breeds, have three genotypes.
This was true even for seemingly insignificant genetic changes known as "silent mutations
," which swap out a single DNA letter without changing the ultimate gene product.
The five silent mutations
p.C6C, p.R450R, p.R723R, p.N570N and p.V632V described previously in Moroccan population (9,10) were also detected in our sample.
PAH exposures were also inconsistently related to p53 missense, nonsense, and silent mutations
However, experiments by Michael Gottesman of the National Cancer Institute in Bethesda, Md., and his colleagues led the team to suspect that those silent mutations
might lead to significant differences.