shuttle vector


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Related to shuttle vector: expression vector, cloning vector

shut·tle vec·tor

a vector that contains both bacterial and eukaryotic replication signals; thus, replication can occur in both types of cells.
See also: vector (4).

shuttle vector

A DNA plasmid with both bacterial and eukaryotic replicative machinery, which allows it to “promiscuously” propagate within bacteria and yeasts. Recombinant DNA in shuttle vectors can be amplified in bacteria and expressed in eukaryotes.

shuttle vector

A short DNA segment, such as may be found in a bacteriophage or a plasmid, that carries DNA between organisms of two different species.
See also: vector

shuttle vector

a VECTOR (2) that is capable of replicating (see REPLICATION in more than one HOST, for example ESCHERICHIA COLI and YEAST.
References in periodicals archive ?
Southern blot analysis was performed to determine whether the shuttle vectors (pFJ1, pFJ4, and pFJ6) had integrated into the J7-F chromosome and to measure their copy numbers.
To construct shuttle vectors for J7 derivatives, oriCs in the J7 chromosome were predicted using the web-based Ori-Finder 2 tool.
To assess the structural stability of all shuttle vectors (pFJ1, pFJ3, pFJ4, pFJ5, pFJ6, and pFJ7) and verify their ability to replicate independently in J7-F cells, plasmid DNA was extracted from each vector transformant and back-transformed into E.
Shuttle vectors containing chromosome oriCs are usually present at one copy per chromosome [36].
Compatible shuttle vectors are excellent tools for investigating protein-protein and protein-DNA interactions in prokaryotes.
Second, the molecular weights of the vectors constructed here were all around 6 kb, which is much smaller than that of most of the archaeal shuttle vectors previously reported [37], including pYCJ (about 9.9 kb).
Six of the shuttle vectors containing one or two of the oriCs could replicate independently in J7-F cells, suggesting that at least six of the 12 predicted oriCs had the ability to initiate DNA replication.
Doolittle, "Shuttle vectors for the archaebacterium Halobacterium volcanii," Proceedings of the National Academy of Sciences of the United States of America, vol.
Dyall-Smith, "Improved shuttle vectors for Haloferax volcanii including a dual-resistance plasmid," Gene, vol.
Shinmura et al., "OGG1 protein suppresses G:C [right arrow] T:A mutation in a shuttle vector containing 8-hydroxyguanine in human cells," Carcinogenesis, vol.
Takebe, "Molecular analysis of mutations induced by 2-chloroacetaldehyde, the ultimate carcinogenic form of vinyl chloride, in human cells using shuttle vectors," Carcinogenesis, vol.