serum-free media


Also found in: Acronyms.

serum-free media

A well-defined and controlled culture media that has been processed in some form (e.g., filtered to remove inhibitors and/or growth factors). Serum-free media are not necessarily protein-free or chemically defined; most are optimised for the growth requirements of cell-specific applications.
References in periodicals archive ?
It will allow us to fulfil the increasing demand for GMP-grade cytokines and serum-free media.
8 in the HELF populations grown in the "Hybris" media supplemented with 2% FBS, while the growth in the serum-free media led to a modest increase by a factor of 3.
HBMECs were incubated with astaxanthin 10 [micro]M for 24 h in 24-well plates in serum-free media previously coated with growth factor-reduced matrigel matrix, and then IWR-1-endo with 5 [micro]M continue to develop 8 h.
Achieving robust differentiation and cell viability in serum-free media provides researchers the consistency and quality required for clinical studies.
To compare the ability of the various PRP products to stimulate proliferation of human bone marrow (BM), rat BM and compact bone (CB)-derived mesenchymal stem cells (MSC), cells were cultured in serum-free media for 4 and 7 days with varying concentrations of PRP, PPP, or combinations of recombinant mitogens.
Growth factors are essential for the long-term growth and proliferation of cell lines in serum-free media formulations.
Further, recent trend is also moving towards use of serum-free media or serum alternatives due to animal welfare and ethical issues in serum processing and production.
The evidence for albumin-bound lipids as modulators of cell physiology is widely available and the importance of this for industrial cell culture in the development of serum-free media has been long recognized.
Several different serum-free media (Table:1) were used to compare their effectiveness on cell growth and protein expression.
When confluent, the cells were exposed for 1, 6, and 24 h to either (a) serum-free media (control) or (b) serum-free media containing different CRP levels (3, 6,12.
They had already developed techniques for growing various nervous system cells in serum-free media, including motoneurons, and here they attempted myelination using the growth medium they have worked with for many years.
These additions to Sigma-Aldrich's growing portfolio of research tools for regenerative medicine are expected to consist of serum-free media for bone marrow and umbilical cord stem cells, mesenchymal stem cells and neural stem cells; cytokine cocktails for stem cell expansion and differentiation; novel formulations for cyropreservation of stem cells; and specialized products to conduct clonogenic assays of the various stem cells (http://www.
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