To compare the efficiency of ammonia-ethanol and AutoFluo Quencher in eliminating AF in FFPE bone marrow tissue, serial sections
from the same patient were treated with ammonia-ethanol or AutoFluo Quencher.
Methods: Serial sections
of 27 human embryonic hearts from Carnegie stage (CS) 10 to CS19 were immunohistochemically stained with antibodies against a-smooth muscle actin (a-SMA) and myosin heavy chain.
Using a microtome, transverse serial sections
were cut at 10m with 5 sections cut 7m thick after every 20 sections.
To define specific cell types, we performed immunohistochemical analysis by using antibodies against neuronal nuclei (Abcam, Cambridge, MA, USA), glial fibrillary acidic protein (Agilent, Santa Clara, CA, USA), and CD163 (Leica Biosystems, Buffalo Grove, IL, USA) on serial sections
of block positive for Zika virus by ISH from selected case-patients, according to previously described protocol (19).
The system incorporates a 300-slide autoloader that supports multiple 1x3 and 2x3 inch slides and registration of serial sections
for 3D reconstruction, along with 6x8 inch slides for large specimen imaging.
(small wedge, defect open; small wedge, defect closed; large wedge, defect open; large wedge, defect closed [equivalent to a protruding ear]; and pinnectomy) were undertaken, and the sound intensity changes assessed....
Three sets of 15 consecutive serial sections
, each measuring 3-5 [micro]m in thickness and mounted on separate slides, were prepared from each case at one sitting, taking care to always use the same microtome in order not to lose any tissue in between sections.
Transverse serial sections
of whole juvenile pearl oysters were prepared.
The tissue blocks were cut horizontally in serial sections
at 4 pm thickness using rotator microtome (Leica, UK).
of cervical and thoracic segments of 6wks.
Typically, researchers mount resin-embedded specimens on the ultramicrotome, cutting serial sections
that then float on a water surface in the diamond knife trough.
Although multiple histologic serial sections
of the brain were examined and a brain squash performed and analyzed, no Baylisascaris larvae were found.