serial passage

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se·ri·al pas·sage

successive transfer of an infectious agent through a series of cultures or experimental animals, usually to attenuate pathogenicity.


introduction followed by recovery of an infectious agent in an experimental animal or culture medium.

blind passage
passage of an infectious agent through an experimental animal or medium without there being any evidence, clinical or cultural, that the agent is present.
serial passage
repeated passage through a series of experimental animals or media, often with the objective of altering the virulence of the agent or adapting it to grow better.
References in periodicals archive ?
VM mice have a different PrP sequence (PrP-b) and have been used to identify BSE after 2 serial passages on the basis of incubation period (IP) and LP data (23).
Additional serial passages from these cattle TSE sources are required for further characterization, especially since the possibility cannot be excluded that transmission of a cattle TSE into mink can modify its biologic properties.
coli by serial passage on cefoxitin-containing medium to determine which of these two resistance mechanisms was predominant and if our findings were representative of those seen in clinical isolates.
Transformants retained resistance till 70th passage ciprofloxacin was found to have moderate activity in stability after 48th number of serial passages.
It has been known that with increasing cell serial passages, BMSCs display the senescence-associated phenotypes as a result of degradation of cellular organelles [15].
Further studies, such as continuous serial passages and neutralization assays, are needed to determine the final activity of the virus in Vero cells.
It is clear from the results that pathogenicity of original IBDV reduced considerably after serial passages in embryonated chicken eggs.
Because DT104o was highly variable, 5 DT104 strains were tested for the frequency of variants at each locus after 5 serial passages by using liquid culture: cultures were diluted 1:1,000 at each passage.
Serial passages of the AIV through embryos improved it's HA and biological titer but tremendously decreased its antigenicity.