serial dilution


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dilution

 [di-loo´shun]
1. reduction of concentration of an active substance by admixture of a neutral agent.
2. a substance that has undergone such a process.
serial dilution a set of dilutions in a mathematical sequence. In microbiological technique, serial dilutions are used to obtain a culture plate that yields a countable number of separate colonies. From this, a calculation of viable cells in the original suspension can be made, as a colony picked for pure culture.

serial dilution

a laboratory technique in which a substance, such as blood serum, is decreased in concentration in a series of proportional amounts. In antibody analysis, for example, a serum sample may be distributed in a series of tubes so that each has one half of the amount of the previous tube in the series, resulting in titers of 1:5, 1:10, 1:20, and so on.

se·ri·al di·lu·tion

(sēr'ē-ăl di-lū'shŭn)
The preparation of a graded set of solutions or suspensions, each member of the set being a fixed dilution (typically 1:2) of the preceding member; used particularly in titration of antibodies in serum.

dilution

1. reduction of concentration of an active substance by admixture of a neutral agent.
2. a substance that has undergone dilution.

limit dilution
a method of obtaining a pure culture of bacteria or virus by subculturing from the highest dilution in which the organism is demonstrably present.
serial dilution
1. the progressive dilution of a substance or infectious agent in a series of tubes or wells in a tray in predetermined ratios, e.g. 2-fold or 10-fold dilution steps.
2. a method of obtaining a pure bacterial culture by rapid transfer of a small amount of material from one nutrient medium to a succeeding one of the same volume.
References in periodicals archive ?
2) Noon actually used serial dilutions of grass pollen extracts, quantifying the response of the conjunctiva to a given allergen.
The effectiveness of Chelex as ah extraction method compared with the Qiagen kit was evaluated by comparing the amplification generated from both extraction methods, using serial dilutions of P.
Observed titres of serum samples were calculated by plotting the average of triplicate, corrected absorbance readings at serial dilutions until the PNT baseline was intersected (Fig.
Semiquantitative CRAG LFA titers were determined on cryopreserved samples by using a 2-fold serial dilution starting at 1:25.
Volumes of the leaf extract are then used to prepare serial dilutions ([10.
I use my automated liquid handler to perform serial dilutions in microtiter plates and I want to understand where the biggest potential for errors would be.
The viable count of bacteria in the supernatant was determined by plating appropriate serial dilutions on nutrient agar plates.
In this lab, we will start with an unknown amount of beads and simulate a serial dilution so that you will be able to visualize what is happening and be able to calculate the number of beads in your original sample.
When their symptoms persisted, they underwent further evaluation and treatment based on the results of serial dilution quantitative intradermal skin testing.
Pre-programmed application templates for Plate Replication, Serial Dilution, Assay Set-up, Hit Picking and DNA/RNA/Protein Purification allow researchers and scientists to begin working with the Sciclone ALH 4000 immediately, and provide the flexibility to easily switch between applications without the hassle and complexity of programming.
Skin endpoint titration (SET) testing, also referred to as serial dilution quantitative intradermal testing, is a modification of intradermal testing that uses specific antigen dilutions to establish the minimum amount of antigen required to produce a positive skin test.