serial dilution


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dilution

 [di-loo´shun]
1. reduction of concentration of an active substance by admixture of a neutral agent.
2. a substance that has undergone such a process.
serial dilution a set of dilutions in a mathematical sequence. In microbiological technique, serial dilutions are used to obtain a culture plate that yields a countable number of separate colonies. From this, a calculation of viable cells in the original suspension can be made, as a colony picked for pure culture.

serial dilution

a laboratory technique in which a substance, such as blood serum, is decreased in concentration in a series of proportional amounts. In antibody analysis, for example, a serum sample may be distributed in a series of tubes so that each has one half of the amount of the previous tube in the series, resulting in titers of 1:5, 1:10, 1:20, and so on.

se·ri·al di·lu·tion

(sēr'ē-ăl di-lū'shŭn)
The preparation of a graded set of solutions or suspensions, each member of the set being a fixed dilution (typically 1:2) of the preceding member; used particularly in titration of antibodies in serum.

dilution

1. reduction of concentration of an active substance by admixture of a neutral agent.
2. a substance that has undergone dilution.

limit dilution
a method of obtaining a pure culture of bacteria or virus by subculturing from the highest dilution in which the organism is demonstrably present.
serial dilution
1. the progressive dilution of a substance or infectious agent in a series of tubes or wells in a tray in predetermined ratios, e.g. 2-fold or 10-fold dilution steps.
2. a method of obtaining a pure bacterial culture by rapid transfer of a small amount of material from one nutrient medium to a succeeding one of the same volume.
References in periodicals archive ?
Thus, in 2010, the quantitative culture method was changed to a protocol using a 100-[micro]L input volume of undiluted CSF culture and five 1:10 serial dilutions (21,22).
2) Noon actually used serial dilutions of grass pollen extracts, quantifying the response of the conjunctiva to a given allergen.
A series of serial dilutions was prepared over five orders of magnitude, ranging from 6,000,000-600 cells per mL, and DNA was immediately extracted.
Because concentration is volume dependent, these three sources of error are directly connected to serial dilutions.
A linear relationship existed between the predicted titres of the positive sera at a single working dilution (1:250) and the corresponding observed titres as determined by the serial dilution.
The most critical step in serial dilution is calculating how much soil was originally diluted, and by how much it was diluted.
Absolute fitness was estimated by regressing the natural logarithm of population density immediately prior to serial dilution against time for each of the ancestral and evolved genotypes in pure culture in the same medium in which they had evolved (see above) but at a variety of different temperatures (see Bennett and Lenski 1993).
3) Choose an amino acid and make a serial dilution to be used in generating a standard curve.
The medicines are produced in what he termed "potencies" by the serial dilution of alcohol extracts of the individual drugs, followed by a process known as "succession," that is, vigorous shaking with impact.
Actinobacteria were cultivated, enumerated, and isolated using serial dilution and spread-plate techniques under normal nutrient and oligotrophic conditions.
One ml sample was taken from each yogurt sample for serial dilution.
Serial dilution of virus suspensions should provide in-tissue culture maintenance or growth medium to define virus titers and CPE observed different days after qualifying negative and positive control.