By extrapolating current findings for the EGP across the human genome for an estimated 24,000 genes (Ewing and Green 2000; International Human Genome Sequencing
Consortium 2004; Lander et al.
The Sanger method routinely sequences DNA segments containing 800 base pairs, while the 454 Life Sciences report describes sequencing
only 100-base-pair segments.
454 Life Sciences is developing novel technologies for rapidly and comprehensively determining the nucleotide sequence,"whole genome sequencing
," of entire genomes.
Chapters that describe the sequencing
effort are interspersed with chapters that tell how genetic data are being used, and all are full of information.
a service laboratory that performs DNA sequencing
and analysis services for academic and commercial clients, is one of several organizations that tested the BigDye XTerminator Kit.
The combination of the NABsys and GeneSpectrum technologies will enable the combined DNA sequencing
platform to sequence DNA orders of magnitude less expensively and with better information content than any technology currently available," said Dr.
In most cases, links in the database connect users directly to individual websites for the project, sequencing
group, or funding agency.
Only in the past few years have Collins and his colleagues focused on sequencing
The technology involves gel sequencing
, which is the primary technique for sequencing
HCV RNA-positive specimens were further characterized by sequencing
parts of the Core/E1 and NS5B regions.
The ability to sequence many genomes completely has been made possible by the enormous reduction of the cost of sequencing
in the past two decades, from tens of dollars per base in the 1980s to a few cents per base today.
The 300 sequencing
machines at the factory in Celera Genomics' headquarters in Rockville, Md.