The diagnosis of an ovotestis is made histologically, requiring the presence of both seminiferous cords
and ovarian follicles with oocytes .
Primary epithelial cords become seminiferous cords
shortly after their separation from germinal epithelium.
A particular strength of this study was its focus on effects on the seminiferous cords
, which were similar in rat and human tissue.
Testicular sections from control mice revealed seminiferous cords
comprised of Sertoli (capitalize "S" in Sertoli throughout) cells that were mainly located at the periphery of the cords.
2000) and aggregation of germ cells in the seminiferous cords (Barlow and Foster 2003; Kleymenova et al.
In E21.5 DBP-exposed animals, clusters were fewer and, on average, 15% of seminiferous cords contained clusters > 3,000 AU, a size rare in controls (Figure 1A).
However in the few seminiferous cords that exhibited germ cell aggregation after exposure to DBP, there was a lack of Sertoli cell cytoplasmic "fingers" extending around the germ cells, again comparable to the findings in rats.
The active phthalates also alter fetal testis seminiferous cords, an effect manifested by the induction of multinucleated germ cells (MNGs) (Boekelheide et al.
Because of the known effects on fetal testes of MEHP, the nonbrominated analog of TBMEHP (Figure 1), we evaluated testes from fetuses of exposed dams for induction of MNGs (a manifestation of altered seminiferous cords) and testosterone production.
MNG formation is a convenient measure of phthalate-induced effects on the seminiferous cords (Boekelheide et al.
KEY WORDS: animal model, fetal testis, human, mouse, multinucleated germ cells, phthalate, rat, seminiferous cords, testicular dysgenesis, xenotransplant.
However, both species exhibited enlarged seminiferous cords and MNG formation.