Figure 3 shows the quantification of CD133 positive stem cells (neoblasts) in the periphery of the
seminiferous tubules of D.
The primary type A diploid spermatogonia lie at the base of the
seminiferous tubules, where they can either renew themselves and/or differentiate into type B spermatogonia (Phillips et al., 2010).
At the end of mating period (late July) the spermatogenesis is over and the
seminiferous epithelium gradually reduces in thickness up to only spg and Sertoli cells remain (Figure 2(a)).
Seminiferous tubules and interstitium in testicular tissue were found to be normal in the control animals.
Regarding the tissue constituents of the testis, many irregularly outlined
seminiferous tubules showed disarranged epithelial layers and necrotic cellular debris, which corroborates previous reports [43, 49].
In this research, rats treated with nicotine demonstrated less mature sperm in the
seminiferous tubule.
A cross section of a
seminiferous tubule of mice exposed to B[a]P at 100mg/kg/day showed apoptosis in germ cells as detected using the TUNEL assay as compared with the control (Figure 6).
The histopathological lesions such as atrophy in
seminiferous tubules; degeneration, desquamation, reduction and disorganisation in germinal cells; and interstitial oedema and capillary congestion were observed in alone CP group (Fig.
The process begins with the spermatogonium, which resides next to the basal lamina of the
seminiferous tubules.
Diameter of
seminiferous tubule was expressed as mean +- standard deviation and the significant difference was determined using one way analysis of variance (ANOVA) followed by post Hoc Tukey test.
The control negative group (200x; H&E) has normal cyto-architecture, reveal interstitial tissues (IT) and
seminiferous tubules (ST) with sustentacular cells and the normal developing germ cells: spermatogonia (Sp), spermatocytes I (SPI), spermatids (Spt), spermatozoa (Spz) with flagellum.
The Johnsen score (13) Score Level of spermatogenesis 1 No
seminiferous epithelial cells, tubular sclerosis 2 No germ cells, Sertoli cells only 3 Spermatogonia only 4 Few spermatocytes, arrest of spermatogenesis at the primary spermatocyte stage 5 Many spermatocytes 6 Few early spermatids, arrest of spermatogenesis at the spermatid stage 7 No late spermatids, many early spermatids 8 Less than five spermatozoa per tubule 9 Slightly impaired spermatogenesis 10 Full spermatogenesis Table 2.
