scanning optical microscopy


Also found in: Acronyms.

scanning optical microscopy

A method of light microscopy in which the object, often a block of wet or dry tissue, is illuminated with a scanned laser beam in such a way as to bring a narrow plane into focus while leaving the remainder out of focus. Serial observations, with photographs, of different planes can be made so that cutting sections is unnecessary. A three-dimensional image can be built up. The sites of binding of fluorescence-labelled antibodies can be determined with great accuracy.
References in periodicals archive ?
Letokhov, "Double-resonance probe for near-field scanning optical microscopy," Review of Scientific Instruments, vol.
Hafner, "Polarization mode coupling due to metal-layer modifications in apertureless near-field scanning optical microscopy probes," J.
Next come contributions discussing confocal scanning laser microscopy, quantitative computer-assisted image analysis, laser scanning cytometry, laser capture microdissection, microarray image scanning, near-field scanning optical microscopy, atomic force microscopy, and reflection contrast microscopy.
SPM encompasses three main techniques: atomic force microscopy (AFM) (see IBO 12/31/94), scanning tunneling microscopy (STM) and near-field scanning optical microscopy (NSOM) (see IBO 5/15/05).
However, near-field scanning optical microscopy (NSOM), with its ability to generate optical micrographs with sub-diffraction limit resolution offers a means to accomplish such measurements.
(19) Chemical imaging with near field scanning optical microscopy. Stephen J.
of Pittsburgh) present examples of the applications of the technology in 17 papers discussing near-field scanning optical microscopy, mechanical studies of polymers by atomic force microscopy, single- molecule studies, and polymer surface characterization by scanned probes.
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