scanning optical microscopy

Also found in: Acronyms.

scanning optical microscopy

A method of light microscopy in which the object, often a block of wet or dry tissue, is illuminated with a scanned laser beam in such a way as to bring a narrow plane into focus while leaving the remainder out of focus. Serial observations, with photographs, of different planes can be made so that cutting sections is unnecessary. A three-dimensional image can be built up. The sites of binding of fluorescence-labelled antibodies can be determined with great accuracy.

References in periodicals archive ?

Letokhov, "Double-resonance probe for near-field scanning optical microscopy," Review of Scientific Instruments, vol.

True Tapping Mode Scanning Near-Field Optical Microscopy with Bent Glass Fiber Probes

Hafner, "Polarization mode coupling due to metal-layer modifications in apertureless near-field scanning optical microscopy probes," J.

Interaction of an asymmetric scanning near field optical microscopy probe with fluorescent molecules

Next come contributions discussing confocal scanning laser microscopy, quantitative computer-assisted image analysis, laser scanning cytometry, laser capture microdissection, microarray image scanning, near-field scanning optical microscopy, atomic force microscopy, and reflection contrast microscopy.

Cell Imaging Techniques: Methods and Protocols

SPM encompasses three main techniques: atomic force microscopy (AFM) (see IBO 12/31/94), scanning tunneling microscopy (STM) and near-field scanning optical microscopy (NSOM) (see IBO 5/15/05).

Microscopy techniques focus on nanotech

However, near-field scanning optical microscopy (NSOM), with its ability to generate optical micrographs with sub-diffraction limit resolution offers a means to accomplish such measurements.

Measuring local birefringence in photonic materials. (General Developments)

(19) Chemical imaging with near field scanning optical microscopy. Stephen J.

Savannah hosts Rubber Division. (Meetings)

of Pittsburgh) present examples of the applications of the technology in 17 papers discussing near-field scanning optical microscopy, mechanical studies of polymers by atomic force microscopy, single- molecule studies, and polymer surface characterization by scanned probes.