We measured plasma TMAO, choline, betaine, and dimethylglycine; serum sarcosine
; and urine choline, betaine, dimethylglycine, and sarcosine
by LC-MS/MS (14) or GC-MS/MS (15).
Cobalamin, betaine, dimethylglycine, sarcosine
, total homocysteine, total cysteine, tryptophan, ADMA, SDMA, creatinine, and methylmalonic acid were essentially stable under all conditions.
The bacterial cells in the agarose plugs were lysed by treatment with lysis solution (1 mg of lysozyme per mL, 0.4% N-sodium lauryl sarcosine
, 0.2% Na-deoxycholate, 10 mM Tris [pH 7.2], 50 mM NaC1) at 37 [degrees] C overnight and treated with proteinase K at 50 [degrees] C overnight.
This method is based on the same reaction sequence as the Creatinine-PAP (phenol aminophenazone) method (29) consisting of 4 consecutive enzymatic steps via creatininase, creatinase, sarcosine
oxidase, and peroxidase, but using a different chromophore (2,4,6-triiodo-3-hydroxybenzoic acid) and a modified detergent composition.
Phase A Lipacide C8G (Seppic) 0.50 (caprylol glycine) Methylparaben 0.10 Ethylparaben 0.025 Propylparaben 0.0125 Butylparaben 0.025 Isobutylparaben 0.0125 Aqua/water 20.00 Disodium EDTA 0.10 TEA 25% 1.45 Phase B Oronal LCG (Seppic) 1.00 (sodium coceth sulfate and PEG-40 glyceryl cocoate) Fragrance 0.10 Phase C Sepicalm S (Seppic) 0.30 (sodium cocoyl amino acids (and) sarcosine
(and) potassium aspartate (and) magnesium aspartate) Water q.s to 100 Lactic acid q.s.
The enzymatic method is based on the determination of sarcosine
after conversion of creatinine with creatininase, creatinase, and sarcosine
INCI name: lecithin (and) butyl stearate (and) cocoyl hydrolyzed collagen (and) oleoyl sarcosine
(and) sesame oil (and) lanolin alcohol
One method was based on coupling of enzymatic bioassays using creatininase, creatinase, and sarcosine
oxidase and electrophoresic separation and amperometric detection of reaction products (46).
Tri-K HMF Complex INCI name: acetamide mea (and) hydrolyzed hair keratin (and) panthenol (and) sorbitol (and) sodium cocoyl collagen amino acid (and) cocoyl sarcosine
(and) wheat germ acid (and) wheat germ oil (and) linolenic acid (and) linoleic acid (and) sulfur (and) polysorbate 80 (and) oleth-10 (and) jojoba oil (and) tocopheryl acetate (and) hydrolyzed glycosaminoglycans Suggested use level: 3-7% Applications: shampoos, moisturizers, conditioners Comments: Tri-K HMF Complex is a moisturizing complex designed to increase moisture in damaged hair.
Biotin was conjugated to a sarcosine
linker that served two functions: (a) it provided the moiety for stable-isotope introduction in the synthesis of internal standards, and (b) it blocked the action of the enzyme biotinidase (15), which is present in biological fluids and could cleave the biotin handle off the product conjugates.
* Seppic Paris, France Tel: 01 40 62 55 59 Fax: 01 40 62 52 53 E-mail: firstname.lastname@example.org Sepicalm S INCI: sodium cocoylaminoacid and sarcosine
and potassium aspartate and magnesium aspartate Applications: suitable for sensitive skins and helps skin to resist external aggressions of a mechanical, chemical of actinic nature (in vivo tested) Use levels: 1 to 3% Sepicalm VG INCI: sodium palmitoylproline and water lily (nymphea alba) extract Applications: A soothing agent able to decrease feeling of discomfort (heat, stinging) felt during sun aggressions.
 Nonstandard abbreviations: 4-NA, 4-nitroanilide; [[alpha].sub.2]-M, [[alpha].sub.2]-macroglobulin; Pip, pipecolic acid; Sar, sarcosine
(N-methyl glycine); CBZ, carbobenzoxy; PEG, polyethylene glycol; and mPEG, methoxypolyethylene glycol.