The extract was treated with doubly distilled water, methanol and 20 % KOH/methanol (1:1:1) to saponify
any triglyceride present.
Saponification value was expressed as the number of mg of potassium hydroxide required to saponify
one gram of oil or fat (AOCS Cd 3-25).
Recipes containing a large percentage of olive oil sometimes take longer to saponify
(chemical jargon for "make soap").
Reactions were stopped by adding 3 mL of 100 g [L.sup.-1] KOH in methanol and the mixtures were heated at 80 [degrees] C for 1 h to saponify
the acyl lipids and acyl-CoAs.
High alkalinity products saponify
fats and vegetable oils into soluble soaps.
Thereafter, 2 mL of 0.66 N of methanolic KOH (R&M Chemicals, Essex, UK) were added to saponify
the lipid extract, and the tubes were capped and transferred to a water bath at 80[degrees]C for 10 min with occasional shaking.