Here, in this study, the influence of hydrogel ionogenity and hydrophilicity on the swelling behavior, adsorption and decolorization of the phenazine dyes such as Neutral Red, Safranin
T, and Janus Green were investigated, too.
Rajabi, "[Fe.sub.3][O.sub.4] magnetic nanoparticles modified with sodium dodecyl sulfate for removal of safranin
O dye from aqueous solutions," Desalination, vol.
In this vein, the magnetite/carbon nanocomposites (MNC) were tested as adsorbents for removal of two anionic dyes, Chromazurol S (ChS) and Nylosan Blue (NB), and a cationic dye, Safranin
T (Basic Red 2, BR2).
(b) Hind paw specimens were collected from recipient mice treated with different types of iTregs on the 49th day after the first immunization with CII and were stained with H&E (top, synovial joint inflammation) and Safranin
O (bottom, cartilage erosion).
(a) Representative mitochondrial [DELTA][[psi].sub.m] traces in permeabilized MCU-silenced cardiomyocytes using 2 [micro]M safranin
, after 7.5 [micro]M [Ca.sup.2+] addition.
O staining showed that proteoglycan was well preserved in the joints of the naive (Figure 3(a): (A)) and LJF-HE-treated (Figure 3(a): (C)) rats, but not in the joints of the control rats, which resulted in complete loss of proteoglycan (no red color) and bone resorption (red color).
Subsequently, paraffin sections (thickness: 5 [micro]m) were prepared and subjected to conventional safranin
O staining (Solarbio, Beijing, China) and haematoxylin and eosin (H&E) staining (Solarbio).
The cross-sections were clarified with a commercial solution of 50% sodium hypochlorite and stained with safranin
and Astra-Blau (Safrablau), according to Bukatsch (1972) modified.
The tubes were then washed once with distilled water and then stained with 1% safranin
. The tubes were then kept still for 7 min.
The final step involved using anti-stain, safranin
for 45 s.
After washing, safranin
was used for counter staining.
Two-Dimensional Image Processing: The sections were stained with hematoxylin for 3 minutes, differentiated in 1% acid alcohol for 15 s, stained again with 0.02% aqueous fast green for 3 minutes,, and counterstained with 0.1% Safranin
O for 3 minutes, as described previously.10 Finally, the sections were dehydrated through a graded alcohol series, cleared in xylene, and mounted onto glass slides using neutral gum.