An additional culture was fed 5000 cells [larva.sup.-1] [ml.sup.-1] in the presence of 100 nmol 1[1.sup.-1] rapamycin, a concentration determined by a survey of the literature to be used routinely to inhibit TOR activity in mammalian cells (e.g., Almilaji et al., 2012), and which we showed in preliminary experiments to inhibit rudiment
development while having no adverse effect on larval somatic growth or survival.
were microdissected from E12.5 C57BL/6J mouse embryos and then pooled and assigned randomly to different experimental groups.
Larvae were sampled daily until the ectodermal invagination contacted the mesoderm in the echinus rudiment
. Larvae were subsequently sampled every 2 d from the high food treatment and every 4 d from the low food and filtered water treatments until they reached the late eight-armed stage.
Developmental studies have begun to show how left-right asymmetry that leads to differential coelomic development and rudiment
formation on the left side is organized in sea urchin larvae.
Immunostaining revealed several skeletogenic cells in a cluster at the site where each skeletal rudiment
of the posterodorsal rod (Fig.
On the lateral sides of the rudiment
, the coelomic epithelium recovers its normal histological structure (Fig.
To improve antiserum penetration, and thus the staining of the rudiment
in older larvae, a further permeabilization step was carried out.
This concept of the effects of hormones on snail reproduction, together with the effects of endocrine disruption in molluscs, allowed me to demonstrate, for the first time, that the so-called "vestigial penis," named more than one hundred years ago (4, 5), is a rudiment
of the penis itself.
It will be interesting to see our media being taught by the 'new masters' from across the West Philippine Sea on the rudiments
of toeing the government line, or learning to polish propaganda pieces to boost the powers-that-be-in other words, to be total robotic trolls like the wonderful Mocha Uson.
These sessions are taking place across the heartland of rugby league and beyond and are giving youngsters a chance to learn the rudiments
of the game whilst having fun as well.
A second section covers applicable real and and complex functions, with such topics as algebra of complex numbers, computation of definite integrals, and rudiments
of the Fourier transform.
STUDENTS at seven Kirklees schools will learn the rudiments
of good business practice whilst raising money for charity.