The seeds were dried on autoclaved filter paper and cultured in triplicate on basal MS medium containing Murashige and Skoog mineral salts, 3% sucrose and 7 g/l agar and incubated in growth chamber at ambient temperature and light conditions to obtain explants (shoot and
root tip, basal plate, leaf blade) for genetic transformation experiments.
Effects of Jatropha curcas oil in Lactuca sativa
root tip bioassays.
The surgeon can now visualize the entire bony crypt, lesion, and
root tip securely and reliably.
A three-mm segment of
root tip was removed and transferred into a microcentifuge tube and stored at -70[degrees]C until extraction.
After the exposure period has elapsed, the bulbs were transferred into clean tap water and
root tip samples from all replicates were obtained at 24h and 44h postexposure.
The coronal two-thirds of the root surface was covered with saline-soaked wet gauze, and the apical 3 mm of the
root tip was sectioned with a diamond point bur (Komet 858; Komet, Rock Hill, SC, USA) under copious irrigation with sterile saline.
Inclusion Criteria: Healthy children aged 3-8 years old who could receive dental treatment under general anesthesia; deciduous molars with deep caries or those close to the site of removed pulp detritus; clinical and imaging examinations revealed deeply carious deciduous molars without pulpal degeneration (without spontaneous pain history, palpation pain, percussion pain, swelling, fistula, pathological movement or alveolar bone destruction at the
root tip); available for prefabricated metal crown (PMC) repair after treatment.
leaf whorl inoculation, stem injection, tooth-pick and
root tip cut and dip.
Other parasitic plants tap directly into their host plants through the use of a modified
root tip known as a haustorium.
cepa
root tip meristem model and compared to a mutagenic drug (sodium azide).
One simulator output was connected to the wire segment that imitated the tooth
root tip, the other output was connected to the passive electrode of the apex locator E2.
About 2-3 mm terminal end of each
root tip was then dissected and directly used for chromosome analysis or transferred into 70% ethyl alcohol and stored at 4[degrees]C for later investigation.
